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PMID: 9287000 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

An lrp-like gene of Bacillus subtilis involved in branched-chain amino acid transport.

Journal of bacteriology ·Vol. 179 ·No. 17 ·1997-09-00 ·Pages 5448-57

Belitsky BR, Gustafsson MC, Sonenshein AL, Von Wachenfeldt C

Abstract

The azlB locus of Bacillus subtilis was defined previously by a mutation conferring resistance to a leucine analog, 4-azaleucine (J. B. Ward, Jr., and S. A. Zahler, J. Bacteriol. 116:727-735, 1973). In this report, azlB is shown to be the first gene of an operon apparently involved in branched-chain amino acid transport. The product of the azlB gene is an Lrp-like protein that negatively regulates expression of the azlBCDEF operon. Resistance to 4-azaleucine in azlB mutants is due to overproduction of AzlC and AzlD, two novel hydrophobic proteins.

MeSH Terms
Amino Acids/analysis Bacillus subtilis/drug effects,genetics,metabolism Bacterial Proteins/chemistry Base Sequence Biological Transport Carrier Proteins/genetics,physiology DNA-Binding Proteins/genetics Drug Resistance, Microbial/genetics Gene Expression Regulation, Bacterial/genetics Genes, Bacterial/genetics Glutamate Synthase/metabolism Leucine/analogs & derivatives,metabolism,pharmacology Leucine-Responsive Regulatory Protein Molecular Sequence Data Open Reading Frames/genetics Operon/genetics Phylogeny Recombinant Fusion Proteins Repressor Proteins Restriction Mapping Sequence Analysis, DNA Transcription Factors
Chemicals
Amino Acids Bacterial Proteins Carrier Proteins DNA-Binding Proteins Recombinant Fusion Proteins Repressor Proteins Transcription Factors Leucine-Responsive Regulatory Protein azaleucine Glutamate Synthase Leucine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Belitsky B R
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.
Gustafsson M C
Sonenshein A L
Von Wachenfeldt C
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.179.17.5448-5457.1997
Published
1997-09-00
Pages
5448-57
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC179416
Subset
IM
Grants
NIGMS NIH HHS · GM36718 · United States
Databases
GENBANK
AB001488, AE000097, D82943, L07247, L34333, M61151, M97860, S52279, U39263, U42216, U67472, U67519, U85623, X78346, X91878, Y08256, Y11043, Z79702, Z92771, Z93767, Z93934
SWISSPROT
P03809, P19494, P42179, P44337, P44580, P45265
External Links
PubMed source DOI: 10.1128/jb.179.17.5448-5457.1997 PMC Full Text View journal details
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