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PMID: 928063 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

5' Caps in hnRNA: absence of m32,2,7G and size distribution of capped molecules.

Nucleic acids research ·Vol. 4 ·No. 10 ·1977-10-00 ·Pages 3357-69

Fernandez-Munoz R, Lavi U, Darnell JE

Abstract

In HeLa cells the "small nuclear" RNA has a cap II 5' structure (8)-- m32,2,7G(5') pppXmpYmp-- where X and Y are 2'0 methylated adenosine and uridine. In contrast hnRNA contains only cap I structures were the 2'0 methylated residue may be any base as was earlier reported for cytoplasmic mRNA (8,9,11). With a clear distinction between the source of these two caps an analysis of the size distribution of capped hnRNA could be performed which revealed over 65% of the capped hnRNA molecules were larger than cytoplasmic mRNA.

MeSH Terms
Cell Nucleus/analysis Guanosine/analysis HeLa Cells/analysis Oligoribonucleotides/analysis RNA, Messenger/analysis RNA, Neoplasm Ribonucleases Ribonucleotides/analysis
Chemicals
Oligoribonucleotides RNA, Messenger RNA, Neoplasm Ribonucleotides Guanosine Ribonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Fernandez-Munoz R
Lavi U
Darnell J E
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27 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/4.10.3357
Published
1977-10-00
Pages
3357-69
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342658
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/4.10.3357 PMC Full Text View journal details
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