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PMID: 1054512 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Short-lived messenger RNA in HeLa cells and its impace on the kinetics of accumulation of cytoplasmic polyadenylate.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 1 ·1975-01-00 ·Pages 389-93

Puckett L, Chambers S, Darnell JE

Abstract

Accumulation of [3H]adenine in the acid-soluble pool and in nuclear and cytoplasmic poly(A) of HeLa cells shows that the nuclear poly(A) rises along a curve similar to that of the acid-soluble pool. By use of a [3H]guanosine pulse-chase experiment in adenine-grown cells, at least 35-50% of the pulse-labeled mRNA was found to have a half-life of about 1-2 hr. A mathematical model involving nuclear poly(A) synthesis and conservative transport to the cytoplasm has been derived from the new information about mRNA with a short half-life. This model predicts curves similar to those found for nuclear and cytoplasmic accumulation of poly(A). Thus, there is no necessity on kinetic grounds to invoke either nuclear turnover or cytoplasmic synthesis of poly(A).

MeSH Terms
Adenine/metabolism Adenine Nucleotides/metabolism Cell Nucleus/metabolism Cytoplasm/metabolism Guanosine/metabolism Half-Life HeLa Cells/metabolism Kinetics Mathematics Models, Biological Polynucleotides/metabolism RNA, Messenger/metabolism RNA, Neoplasm/metabolism Tritium
Chemicals
Adenine Nucleotides Polynucleotides RNA, Messenger RNA, Neoplasm Tritium Guanosine Adenine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Puckett L
Chambers S
Darnell J E
References (24)
24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.1.389
Published
1975-01-00
Pages
389-93
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432311
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.1.389 PMC Full Text View journal details
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