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PMID: 9278540 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Axonal transport blockade in the neonatal rat optic nerve induces limited retinal ganglion cell death.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 17 ·No. 18 ·1997-09-15 ·Pages 7045-52

Fagiolini M, Caleo M, Strettoi E, Maffei L

Abstract

Optic nerve section in the newborn rat results in a rapid apoptotic degeneration of most axotomized retinal ganglion cells (RGCs). This massive process of neuronal death has been ascribed mainly to the interruption of a trophic factor supply from target structures rather than to the axonal damage per se. To distinguish between these two possibilities, we induced a reversible axonal transport blockade in the developing optic nerve by topical application of a local anesthetic (lidocaine). Light and electron microscopy showed no alterations in the fine structure of treated optic nerves. Retinae of treated and control rats were stained with cresyl violet and examined at different times after surgery. We found that axonal transport blockade induced only a limited number of pyknotic RGCs. Degeneration of these cells was completely prevented by inhibiting protein synthesis during lidocaine application. We conclude that the rapid degeneration of RGCs after axotomy can be ascribed only partly to the loss of retrogradely transported trophic factors.

MeSH Terms
Anesthetics, Local/pharmacology Animals Apoptosis Axonal Transport/drug effects,physiology Axons/physiology Cycloheximide/pharmacology Lidocaine/pharmacology Optic Nerve/growth & development Protein Synthesis Inhibitors/pharmacology Rats Retinal Ganglion Cells/cytology,drug effects
Chemicals
Anesthetics, Local Protein Synthesis Inhibitors Cycloheximide Lidocaine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Fagiolini M
Scuola Normale Superiore and Istituto di Neurofisiologia del Consiglio Nazionale delle Ricerche, 56127 Pisa, Italy.
Caleo M
Strettoi E
Maffei L
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
0270-6474
Published
1997-09-15
Pages
7045-52
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6573284
Subset
IM
Grants
Telethon · 934 · Italy
External Links
PubMed source PMC Full Text View journal details
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