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PMID: 9276951 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Construction of a Lotus japonicus late nodulin expressed sequence tag library and identification of novel nodule-specific genes.

Plant physiology ·Vol. 114 ·No. 4 ·1997-08-00 ·Pages 1335-46

Szczyglowski K, Hamburger D, Kapranov P, de Bruijn FJ

Abstract

A range of novel expressed sequence tags (ESTs) associated with late developmental events during nodule organogenesis in the legume Lotus japonicus were identified using mRNA differential display; 110 differentially displayed polymerase chain reaction products were cloned and analyzed. Of 88 unique cDNAs obtained, 22 shared significant homology to DNA/protein sequences in the respective databases. This group comprises, among others, a nodule-specific homolog of protein phosphatase 2C, a peptide transporter protein, and a nodule-specific form of cytochrome P450. RNA gel-blot analysis of 16 differentially displayed ESTs confirmed their nodule-specific expression pattern. The kinetics of mRNA accumulation of the majority of the ESTs analyzed were found to resemble the expression pattern observed for the L. japonicus leghemoglobin gene. These results indicate that the newly isolated molecular markers correspond to genes induced during late developmental stages of L. japonicus nodule organogenesis and provide important, novel tools for the study of nodulation.

MeSH Terms
Amino Acid Sequence Base Sequence DNA, Complementary Fabaceae/genetics Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Membrane Proteins Molecular Sequence Data Nucleic Acid Hybridization Plant Proteins/genetics Plant Roots Plants, Medicinal Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Sequence Tagged Sites
Chemicals
DNA, Complementary Membrane Proteins Plant Proteins nodulin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Szczyglowski K
Department of Energy Plant Research Laboratory, Michigan State University, East Lansing 48824, USA. szczyglw@pilot.msu.edu
Hamburger D
Kapranov P
de Bruijn F J
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.114.4.1335
Email
szczyglw@pilot.msu.edu
Published
1997-08-00
Pages
1335-46
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC158426
Subset
IM
Databases
GENBANK
AF000382, AF000383, AF000384, AF000385, AF000386, AF000387, AF000388, AF000389, AF000390, AF000391, AF000392, AF000393, AF000394, AF000395, AF000396, AF000397, AF000398, AF000399, AF000400, AF000401, AF000402, AF000403, AF000404, AF000405, AF000406, AF000407, AF000408
External Links
PubMed source DOI: 10.1104/pp.114.4.1335 PMC Full Text View journal details
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