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PMID: 7647567 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A nodule-specific gene encoding a subtilisin-like protease is expressed in early stages of actinorhizal nodule development.

The Plant cell ·Vol. 7 ·No. 6 ·1995-06-00 ·Pages 785-94

Ribeiro A, Akkermans AD, van Kammen A, Bisseling T, Pawlowski K

Abstract

To identify genes specifically expressed during early stages of actinorhizal nodule development, a cDNA library made from poly(A) RNA from root nodules of Alnus glutinosa was screened differentially with nodule and root cDNA, respectively. Seven nodule-enhanced and four nodule-specific cDNA clones were isolated. By using in situ hybridization, two of the nodule-specific cDNAs were shown to be expressed at the highest levels in infected cells before the onset of nitrogen fixation; one of them, ag12 (A. glutinosa), was examined in detail. Sequencing showed that ag12 codes for a serine protease of the subtilisin (EC 3.4.21.14) family. Subtilisins previously appeared to be limited to microorganisms. However, subtilisin-like serine proteases have recently been found in archaebacteria, fungi, and yeasts as well as in mammals; a plant subtilisin has also been sequenced. In yeast and mammals, subtilases are responsible for processing peptide hormones. A homolog of ag12, ara12, was identified in Arabidopsis; it was expressed in all organs, and its expression levels were highest during silique development. Hence, our study shows that subtilases are also involved in both symbiotic and nonsymbiotic processes in plant development.

Related Genes
ag12 ara12
MeSH Terms
Amino Acid Sequence Arabidopsis/genetics Arabidopsis Proteins Gene Expression Regulation, Plant Genes, Plant Mitosporic Fungi Molecular Sequence Data Nitrogen Fixation Plant Proteins/genetics,metabolism Plant Roots/enzymology,growth & development,microbiology Sequence Homology, Amino Acid Subtilisins/genetics,metabolism Symbiosis Trees
Chemicals
Arabidopsis Proteins Plant Proteins Subtilisins ag12 protein, Alnus glutinosa ara12 protein, Arabidopsis
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Ribeiro A
Department of Molecular Biology, Agricultural University, Wageningen, The Netherlands.
Akkermans A D
van Kammen A
Bisseling T
Pawlowski K
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.7.6.785
Published
1995-06-00
Pages
785-94
Language
English
Region
England
NLM ID
9208688
PMCID
PMC160833
Subset
IM
Databases
GENBANK
X85974, X85975
External Links
PubMed source DOI: 10.1105/tpc.7.6.785 PMC Full Text View journal details
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