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PMID: 9271430 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A role for the ETS domain transcription factor PEA3 in myogenic differentiation.

Molecular and cellular biology ·Vol. 17 ·No. 9 ·1997-09-00 ·Pages 5550-8

Taylor JM, Dupont-Versteegden EE, Davies JD, Hassell JA, Houlé JD, Gurley CM, Peterson CA

Abstract

Activation of adult myoblasts called satellite cells during muscle degeneration is an important aspect of muscle regeneration. Satellite cells are believed to be the only myogenic stem cells in adult skeletal muscle and the source of regenerating muscle fibers. Upon activation, satellite cells proliferate, migrate to the site of degeneration, and become competent to fuse and differentiate. We show here that the transcription factor polyomavirus enhancer activator 3 (PEA3) is expressed in adult myoblasts in vitro when they are proliferative and during the early stages of differentiation. Overexpression of PEA3 accelerates differentiation, whereas blocking of PEA3 function delays myoblast fusion. PEA3 activates gene expression following binding to the ets motif most efficiently in conjunction with the transcription factor myocyte enhancer factor 2 (MEF2). In vivo, PEA3 is expressed in satellite cells only after muscle degeneration. Taken together, these results suggest that PEA3 is an important regulator of activated satellite cell function.

MeSH Terms
Animals Binding Sites Cell Differentiation Cloning, Molecular DNA/metabolism DNA-Binding Proteins/metabolism Gene Expression MEF2 Transcription Factors Mice Muscle Fibers, Skeletal/physiology Muscle, Skeletal/cytology,physiology Muscles/cytology,physiology Myogenic Regulatory Factors Regeneration Transcription Factors/genetics,metabolism,physiology Transcriptional Activation
Chemicals
DNA-Binding Proteins MEF2 Transcription Factors Myogenic Regulatory Factors Transcription Factors transcription factor PEA3 DNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Taylor J M
Department of Medicine and Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock 72205, USA.
Dupont-Versteegden E E
Davies J D
Hassell J A
Houlé J D
Gurley C M
Peterson C A
References (57)
57 references, click to expand
  1. The muscle satellite cell: a review.
    Int Rev Cytol. 1984;87:225-51 PMID: 6370890
  2. Transcriptional regulation by extracellular signals: mechanisms and specificity.
    Cell. 1995 Jan 27;80(2):199-211 PMID: 7834740
  3. In situ detection of sequence-specific DNA binding activity specified by a recombinant bacteriophage.
    Genes Dev. 1988 Jul;2(7):801-6 PMID: 3061875
  4. PEA3 is a nuclear target for transcription activation by non-nuclear oncogenes.
    EMBO J. 1989 Nov;8(11):3371-8 PMID: 2555163
  5. Migration of myoblasts across basal lamina during skeletal muscle development.
    Nature. 1990 May 24;345(6273):350-3 PMID: 2111464
  6. The collagenase gene promoter contains a TPA and oncogene-responsive unit encompassing the PEA3 and AP-1 binding sites.
    EMBO J. 1990 Jul;9(7):2241-6 PMID: 2162765
  7. Negative control of the helix-loop-helix family of myogenic regulators in the NFB mutant.
    Cell. 1990 Aug 10;62(3):493-502 PMID: 1696180
  8. Regulation of the myoblast-specific expression of the human beta-enolase gene.
    J Biol Chem. 1995 Feb 10;270(6):2535-40 PMID: 7852315
  9. Myogenin and MEF2 function synergistically to activate the MRF4 promoter during myogenesis.
    Mol Cell Biol. 1995 May;15(5):2707-18 PMID: 7739551
  10. Regulatory role of MEF2D in serum induction of the c-jun promoter.
    Mol Cell Biol. 1995 Jun;15(6):2907-15 PMID: 7760790
  11. MyoD protein accumulates in satellite cells and is neurally regulated in regenerating myotubes and skeletal muscle fibers.
    Dev Dyn. 1995 Mar;202(3):244-54 PMID: 7780174
  12. Stimulation of urokinase expression by TNF-alpha requires the activation of binding sites for the AP-1 and PEA3 transcription factors.
    Biochim Biophys Acta. 1995 Jul 20;1268(1):65-72 PMID: 7626664
  13. A direct physical association between ETS and AP-1 transcription factors in normal human T cells.
    Immunity. 1995 Aug;3(2):223-37 PMID: 7648395
  14. Molecular characterization of the ets-related human transcription factor ER81.
    Oncogene. 1995 Aug 17;11(4):771-9 PMID: 7651741
  15. Phosphorylation of transcription factor p62TCF by MAP kinase stimulates ternary complex formation at c-fos promoter.
    Nature. 1992 Jul 30;358(6385):414-7 PMID: 1322499
  16. ERM, a PEA3 subfamily of Ets transcription factors, can cooperate with c-Jun.
    J Biol Chem. 1995 Oct 6;270(40):23795-800 PMID: 7559555
  17. Transcription of the human beta enolase gene (ENO-3) is regulated by an intronic muscle-specific enhancer that binds myocyte-specific enhancer factor 2 proteins and ubiquitous G-rich-box binding factors.
    Mol Cell Biol. 1995 Nov;15(11):5991-6002 PMID: 7565752
  18. A metalloprotease-disintegrin participating in myoblast fusion.
    Nature. 1995 Oct 19;377(6550):652-6 PMID: 7566181
  19. Involvement of a mitogen-activated protein kinase signaling pathway in the regulation of urokinase promoter activity by c-Ha-ras.
    J Biol Chem. 1995 Sep 29;270(39):23007-12 PMID: 7559439
  20. Role of insulin-like growth factors and myogenin in the altered program of proliferation and differentiation in the NFB4 mutant muscle cell line.
    Proc Natl Acad Sci U S A. 1995 Nov 21;92(24):10874-8 PMID: 7479901
  21. Cooperative activation of muscle gene expression by MEF2 and myogenic bHLH proteins.
    Cell. 1995 Dec 29;83(7):1125-36 PMID: 8548800
  22. The Ets family of proteins: weak modulators of gene expression in quest for transcriptional partners.
    Crit Rev Oncog. 1994;5(6):615-38 PMID: 8519813
  23. Ras-mediated phosphorylation of a conserved threonine residue enhances the transactivation activities of c-Ets1 and c-Ets2.
    Mol Cell Biol. 1996 Feb;16(2):538-47 PMID: 8552081
  24. MafB is an interaction partner and repressor of Ets-1 that inhibits erythroid differentiation.
    Cell. 1996 Apr 5;85(1):49-60 PMID: 8620536
  25. Transcription factor PEA3 participates in the induction of urokinase plasminogen activator transcription in murine keratinocytes stimulated with epidermal growth factor or phorbol-ester.
    Nucleic Acids Res. 1990 Sep 11;18(17):5009-17 PMID: 2119494
  26. The ETS-domain: a new DNA-binding motif that recognizes a purine-rich core DNA sequence.
    Genes Dev. 1990 Sep;4(9):1451-3 PMID: 2253872
  27. The c-Ets oncoprotein activates the stromelysin promoter through the same elements as several non-nuclear oncoproteins.
    EMBO J. 1991 May;10(5):1127-34 PMID: 1850695
  28. Human SRF-related proteins: DNA-binding properties and potential regulatory targets.
    Genes Dev. 1991 Dec;5(12A):2327-41 PMID: 1748287
  29. Ets-related protein Elk-1 is homologous to the c-fos regulatory factor p62TCF.
    Nature. 1991 Dec 19-26;354(6354):531-4 PMID: 1722028
  30. Identification of skeletal muscle precursor cells in vivo by use of MyoD1 and myogenin probes.
    Cell Tissue Res. 1992 Jan;267(1):99-104 PMID: 1310442
  31. Characterization of SAP-1, a protein recruited by serum response factor to the c-fos serum response element.
    Cell. 1992 Feb 7;68(3):597-612 PMID: 1339307
  32. MEF2B is a potent transactivator expressed in early myogenic lineages.
    Mol Cell Biol. 1996 Jul;16(7):3814-24 PMID: 8668199
  33. The activity of the Ets transcription factor PEA3 is regulated by two distinct MAPK cascades.
    Oncogene. 1996 Sep 19;13(6):1323-33 PMID: 8808707
  34. Regulation of muscle differentiation by the MEF2 family of MADS box transcription factors.
    Dev Biol. 1995 Nov;172(1):2-14 PMID: 7589800
  35. MyoD and myogenin are coexpressed in regenerating skeletal muscle of the mouse.
    Dev Dyn. 1992 Jan;193(1):34-9 PMID: 1311614
  36. Molecular cloning and characterization of PEA3, a new member of the Ets oncogene family that is differentially expressed in mouse embryonic cells.
    Genes Dev. 1992 Mar;6(3):481-96 PMID: 1547944
  37. A potential fusion peptide and an integrin ligand domain in a protein active in sperm-egg fusion.
    Nature. 1992 Mar 19;356(6366):248-52 PMID: 1552944
  38. Expression of a 91-kilodalton PEA3-binding protein is down-regulated during differentiation of F9 embryonal carcinoma cells.
    Mol Cell Biol. 1992 May;12(5):2213-21 PMID: 1569949
  39. Interaction of murine ets-1 with GGA-binding sites establishes the ETS domain as a new DNA-binding motif.
    Genes Dev. 1992 Jun;6(6):975-90 PMID: 1592264
  40. Beta-enolase is a marker of human myoblast heterogeneity prior to differentiation.
    Dev Biol. 1992 Jun;151(2):626-9 PMID: 1339335
  41. Enhanced cell-free transcription of the ribosomal protein L32 gene by the polyoma virus enhancer PEA3 DNA-binding protein.
    Eur J Biochem. 1992 Jul 1;207(1):195-200 PMID: 1321041
  42. The ets gene family.
    Cell Growth Differ. 1992 May;3(5):327-34 PMID: 1633115
  43. Human myocyte-specific enhancer factor 2 comprises a group of tissue-restricted MADS box transcription factors.
    Genes Dev. 1992 Sep;6(9):1783-98 PMID: 1516833
  44. Involvement of AP1 and PEA3 binding sites in the regulation of murine tissue inhibitor of metalloproteinases-1 (TIMP-1) transcription.
    Biochim Biophys Acta. 1992 Nov 15;1171(1):41-55 PMID: 1420363
  45. Monoclonal antibodies to the myogenic regulatory protein MyoD1: epitope mapping and diagnostic utility.
    Cancer Res. 1992 Dec 1;52(23):6431-9 PMID: 1384962
  46. The Ets family of transcription factors.
    Eur J Biochem. 1993 Jan 15;211(1-2):7-18 PMID: 8425553
  47. hMEF2C gene encodes skeletal muscle- and brain-specific transcription factors.
    Mol Cell Biol. 1993 Apr;13(4):2564-77 PMID: 8455629
  48. Myocyte enhancer factor (MEF) 2C: a tissue-restricted member of the MEF-2 family of transcription factors.
    Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):5282-6 PMID: 8506376
  49. Specificities of protein-protein and protein-DNA interaction of GABP alpha and two newly defined ets-related proteins.
    Genes Dev. 1992 Dec;6(12B):2502-12 PMID: 1340465
  50. Gene regulation by Ets proteins.
    Biochim Biophys Acta. 1993 Dec 23;1155(3):346-56 PMID: 8268191
  51. A fourth human MEF2 transcription factor, hMEF2D, is an early marker of the myogenic lineage.
    Development. 1993 Aug;118(4):1095-106 PMID: 8269842
  52. A Mef2 gene that generates a muscle-specific isoform via alternative mRNA splicing.
    Mol Cell Biol. 1994 Mar;14(3):1647-56 PMID: 8114702
  53. Molecular cloning and characterization of human ERM, a new member of the Ets family closely related to mouse PEA3 and ER81 transcription factors.
    Oncogene. 1994 May;9(5):1397-406 PMID: 8152800
  54. Ternary complex factors: growth factor regulated transcriptional activators.
    Curr Opin Genet Dev. 1994 Feb;4(1):96-101 PMID: 8193547
  55. Ras/MAP kinase-dependent and -independent signaling pathways target distinct ternary complex factors.
    Genes Dev. 1994 Aug 1;8(15):1803-16 PMID: 7958858
  56. Activation of the myogenic lineage by MEF2A, a factor that induces and cooperates with MyoD.
    Science. 1994 Nov 18;266(5188):1236-40 PMID: 7973707
  57. Identification and purification of a sperm surface protein with a potential role in sperm-egg membrane fusion.
    J Cell Biol. 1987 Jan;104(1):141-9 PMID: 3793758
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.17.9.5550
Published
1997-09-00
Pages
5550-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC232403
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.17.9.5550 PMC Full Text View journal details
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