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PMID: 9260957 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Transcriptional and mutational analyses of the rpoN operon in Caulobacter crescentus.

Journal of bacteriology ·Vol. 179 ·No. 16 ·1997-08-00 ·Pages 5138-47

Janakiraman RS, Brun YV

Abstract

The alternative sigma factor sigma54 is required for the biogenesis of both the flagellum and the stalk in Caulobacter crescentus. The DNA sequence downstream of the sigma54 gene (rpoN) has been determined, revealing three open reading frames (ORFs) encoding peptides of 203, 208, and 159 amino acids. ORF208 and ORF159 are homologous to ORFs found downstream of rpoN in other microorganisms. The organization of this region in C. crescentus is similar to that in other bacteria, with the exception of an additional ORF, ORF203, immediately downstream from rpoN. There is a single temporally regulated promoter that drives the expression of both rpoN and ORF203. Promoter probe analysis indicates the presence of another promoter downstream from ORF203 which exhibits a temporal control that is different from that of the rpoN promoter. Mutational analysis was used to address the function of the proteins encoded by these three downstream ORFs. The mutations have no effect on the transcription of previously known sigma54-dependent flagellar promoters except for a slight effect of an ORF159 mutation on transcription of fljK.

MeSH Terms
Amino Acid Sequence Base Sequence Caulobacter crescentus/genetics,growth & development Cell Cycle DNA Mutational Analysis DNA-Binding Proteins DNA-Directed RNA Polymerases/chemistry,genetics Flagella/genetics,metabolism Molecular Sequence Data Mutagenesis, Insertional Open Reading Frames Operon Phenotype Promoter Regions, Genetic RNA Polymerase Sigma 54 Sequence Alignment Sequence Analysis, DNA Sigma Factor/chemistry,genetics Transcription, Genetic
Chemicals
DNA-Binding Proteins Sigma Factor DNA-Directed RNA Polymerases RNA Polymerase Sigma 54
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Janakiraman R S
Department of Biology, Indiana University, Bloomington 47405, USA.
Brun Y V
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.179.16.5138-5147.1997
Published
1997-08-00
Pages
5138-47
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC179373
Subset
IM
Grants
NIGMS NIH HHS · R01 GM051986 · United States
NIGMS NIH HHS · GM07757 · United States
NIGMS NIH HHS · GM51986 · United States
Databases
GENBANK
AF006743, D90908, M59243, M61753, X16474, X71024
SWISSPROT
P28613, P29960, P30335, P31221, P31222
External Links
PubMed source DOI: 10.1128/jb.179.16.5138-5147.1997 PMC Full Text View journal details
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