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PMID: 1417413 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence of the rpoN (hno) gene region of Alcaligenes eutrophus: evidence for a conserved gene cluster.

Archives of microbiology ·Vol. 158 ·No. 2 ·1992-00-00 ·Pages 107-14

Warrelmann J, Eitinger M, Schwartz E, Römermann D, Friedrich B

Abstract

The nucleotide sequence of the rpoN gene, formerly designated hno, and flanking DNA regions of the aerobic hydrogen bacterium Alcaligenes eutrophus has been determined; rpoN codes for the RNA polymerase sigma factor sigma 54 involved in nitrogen regulation and diverse physiological functions of gram-negative bacteria. In A. eutrophus hydrogen metabolism is under control of rpoN. The Tn5-Mob insertion in a previously isolated pleiotropic mutant was mapped within the rpoN gene. The derived amino acid sequence of the A. eutrophus RpoN protein shows extensive homology to the RpoN proteins of other organisms. Sequencing revealed four other open reading frames: one upstream (ORF280) and three downstream (ORF130, ORF99 and ORF greater than 54) of the rpoN gene. A similar arrangement of homologous ORFs is found in the rpoN regions of other bacteria and is indicative of a conserved gene cluster.

MeSH Terms
Alcaligenes/genetics Amino Acid Sequence Base Sequence Chromosome Mapping DNA-Binding Proteins DNA-Directed RNA Polymerases Gene Expression Regulation, Bacterial Genes, Bacterial/genetics Genetic Variation Hydrogen/metabolism Molecular Sequence Data Multigene Family/genetics Mutagenesis, Insertional RNA Polymerase Sigma 54 Reading Frames/genetics Sequence Homology Sigma Factor/genetics
Chemicals
DNA-Binding Proteins Sigma Factor Hydrogen DNA-Directed RNA Polymerases RNA Polymerase Sigma 54
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Warrelmann J
Institut für Pflanzenphysiologie und Mikrobiologie, Freien Universität Berlin, Federal Republic of Germany.
Eitinger M
Schwartz E
Römermann D
Friedrich B
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Article Info
Journal
Archives of microbiology
Abbr.
Arch Microbiol
ISSN
0302-8933
DOI
10.1007/BF00245213
Published
1992-00-00
Pages
107-14
Language
English
Region
Germany
NLM ID
0410427
Subset
IM
Databases
GENBANK
X66633
External Links
PubMed source DOI: 10.1007/BF00245213 View journal details
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