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PMID: 92571 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Mutant of B-tropic murine leukemia virus synthesizing an altered polymerase molecule.

Journal of virology ·Vol. 31 ·No. 3 ·1979-09-00 ·Pages 741-51

Gerwin BI, Rein A, Levin JG, Bassin RH, Benjers BM, Kashmiri SV, Hopkins D, O'Neill BJ

Abstract

A nonconditional mutant of B-tropic murine leukemia virus (MuLV), defective in polymerase, has been isolated by cloning chronically infected cells. The cell clone containing the mutant produced virus particles which were noninfectious. However, superinfection of the cells by replication-competent XC-negative viruses resulted in the rescue of virus capable of forming plaques in a modified XC test, termed the "complementation plaque assay" (A. Rein and R. H. Bassin, J. Virol. 28:656-660, 1978). Analysis of the noninfectious virions produced without superinfection demonstrated that they contained only 2 to 5% of the wild-type level of reverse transcriptase activity. Purification of this activity indicated that it was associated with a smaller molecule than that produced by wild-type virus. Cells producing the mutant virions did not contain the gag-pol precursor, Pr180gag-pol; however the cells contained proteins of 147K and 114K daltons precipitable with anti-pol serum. All of the normal structural proteins as well as 70S genomic RNA could be detected in the mutant particles. An interference test indicated that a functional ecotropic glycoprotein was synthesized by the mutant. These results indicate that the mutant has a unique defect in the pol gene.

MeSH Terms
Animals Cell Line Clone Cells Defective Viruses/genetics,growth & development Enzyme Precursors/genetics Fibroblasts Mice Moloney murine leukemia virus/analysis,genetics,growth & development Mutation RNA, Viral/analysis RNA-Directed DNA Polymerase/genetics Viral Proteins/analysis Virus Replication
Chemicals
Enzyme Precursors RNA, Viral Viral Proteins RNA-Directed DNA Polymerase
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Gerwin B I
Rein A
Levin J G
Bassin R H
Benjers B M
Kashmiri S V
Hopkins D
O'Neill B J
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.31.3.741-751.1979
Published
1979-09-00
Pages
741-51
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC353502
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.31.3.741-751.1979 PMC Full Text View journal details
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