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PMID: 9251790 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The monovalent cation "leak" transport in human erythrocytes: an electroneutral exchange process.

Biophysical journal ·Vol. 73 ·No. 2 ·1997-08-00 ·Pages 733-45

Richter S, Hamann J, Kummerow D, Bernhardt I

Abstract

The mechanism of the "ground permeability" of the human erythrocyte membrane for K+ and Na+ was investigated with respect to a possible involvement of a previously unidentified specific transport pathway, because earlier studies showed that it cannot be explained on the basis of simple electrodiffusion. In particular, we analyzed and described the increase in the (ouabain+bumetanide+EGTA)-insensitive unidirectional K+ and Na+ influxes as well as effluxes (defined as "leak" fluxes) observed in erythrocytes suspended in low-ionic-strength media. Using a carrier-type model and taking into account the influence of the ionic strength on the outer surface potential according to the Gouy-Chapman theory (i.e., the ion concentration near the membrane surface), we are able to describe the altered "leak" fluxes as an electroneutral process. In addition, we can show indirectly that this electroneutral flux is due to an exchange of monovalent cations with protons. This pathway is different from the amiloride-sensitive Na+/H+ exchanger present in the human red blood cell membrane and can be characterized as a K+(Na+)/H+ exchanger.

MeSH Terms
Binding Sites Bumetanide/pharmacology Cations, Monovalent/blood Egtazic Acid/pharmacology Erythrocyte Membrane/drug effects,metabolism Erythrocytes/drug effects,metabolism Humans In Vitro Techniques Kinetics Membrane Potentials Models, Biological Osmolar Concentration Ouabain/pharmacology Potassium/blood Sodium/blood Sodium-Hydrogen Exchangers/blood
Chemicals
Cations, Monovalent Sodium-Hydrogen Exchangers Bumetanide Egtazic Acid Ouabain Sodium Potassium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Richter S
Biophysics Section, Humboldt University Berlin, Germany.
Hamann J
Kummerow D
Bernhardt I
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(97)78106-2
Published
1997-08-00
Pages
733-45
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1180970
Subset
IM
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1016/S0006-3495(97)78106-2 PMC Full Text View journal details
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