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PMID: 9251780 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Molecular motions within the pore of voltage-dependent sodium channels.

Biophysical journal ·Vol. 73 ·No. 2 ·1997-08-00 ·Pages 603-13

Bénitah JP, Ranjan R, Yamagishi T, Janecki M, Tomaselli GF, Marban E

Abstract

The pores of ion channel proteins are often modeled as static structures. In this view, selectivity reflects rigidly constrained backbone orientations. Such a picture is at variance with the generalization that biological proteins are flexible, capable of major internal motions on biologically relevant time scales. We tested for motions in the sodium channel pore by systematically introducing pairs of cysteine residues throughout the pore-lining segments. Two distinct pairs of residues spontaneously formed disulfide bonds bridging domains I and II. Nine other permutations, involving all four domains, were capable of disulfide bonding in the presence of a redox catalyst. The results are inconsistent with a single fixed backbone structure for the pore; instead, the segments that line the permeation pathway appear capable of sizable motions.

MeSH Terms
Amino Acid Sequence Animals Calorimetry Cysteine Disulfides Female Hydrogen Bonding Kinetics Membrane Potentials Models, Molecular Muscle, Skeletal/physiology Mutagenesis, Site-Directed Oocytes/physiology Point Mutation Potassium Channels/biosynthesis,chemistry,physiology Protein Conformation Rats Recombinant Proteins/biosynthesis,chemistry Xenopus laevis
Chemicals
Disulfides Potassium Channels Recombinant Proteins Cysteine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Bénitah J P
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
Ranjan R
Yamagishi T
Janecki M
Tomaselli G F
Marban E
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
Published
1997-08-00
Pages
603-13
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1180960
Subset
IM
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