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PMID: 8552626 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Structure of the sodium channel pore revealed by serial cysteine mutagenesis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 93 ·No. 1 ·1996-01-09 ·Pages 300-4

Pérez-García MT, Chiamvimonvat N, Marban E, Tomaselli GF

Abstract

The pores of voltage-gated cation channels are formed by four intramembrane segments that impart selectivity and conductance. Remarkably little is known about the higher order structure of these critical pore-lining or P segments. Serial cysteine mutagenesis reveals a pattern of side-chain accessibility that contradicts currently favored structural models based on alpha-helices or beta-strands. Like the active sites of many enzymes of known structure, the sodium channel pore consists of irregular loop regions.

MeSH Terms
Amino Acid Sequence Animals Cadmium/pharmacology Cysteine/chemistry Ion Channel Gating/drug effects Molecular Sequence Data Mutagenesis, Site-Directed Oocytes Protein Structure, Secondary Rats Sodium Channels/chemistry,drug effects Structure-Activity Relationship Tetrodotoxin/pharmacology Xenopus laevis
Chemicals
Sodium Channels Cadmium Tetrodotoxin Cysteine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Pérez-García M T
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Chiamvimonvat N
Marban E
Tomaselli G F
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.93.1.300
Published
1996-01-09
Pages
300-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC40226
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.93.1.300 PMC Full Text View journal details
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