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PMID: 9250678 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Human and Xenopus cDNAs encoding budding yeast Cdc7-related kinases: in vitro phosphorylation of MCM subunits by a putative human homologue of Cdc7.

The EMBO journal ·Vol. 16 ·No. 14 ·1997-07-16 ·Pages 4340-51

Sato N, Arai K, Masai H

Abstract

Saccharomyces cerevisiae Cdc7 kinase is essential for initiation of DNA replication, and Hsk1, a related kinase of Schizosaccharomyces pombe, is also required for DNA replication of fission yeast cells. We report here cDNAs encoding Cdc7-related kinases from human and Xenopus (huCdc7 and xeCdc7, respectively). The cloned cDNA for huCdc7 contains an open reading frame consisting of 574 amino acids with a predicted molecular weight of 63,847 that possesses overall amino acid identity of 32% (54% including similar residues) to Cdc7 and Hsk1. huCDC7 is transcribed in the various tissues examined, but most abundantly in testis. Three transcripts of 4.4, 3.5 and 2.4 kb in length are detected. The 3.5 kb transcript is the most predominant and is expressed in all the tissues examined. A cDNA containing a 91 nucleotide insertion at the N-terminal region of huCDC7 is also detected, suggesting the presence of multiple splicing variants. The huCdc7 protein is expressed at a constant level during the mitotic cell cycle and is localized primarily in nuclei in interphase and distributed diffusibly in cytoplasm in the mitotic phase. The wild-type huCdc7 protein expressed in COS7 cells phosphorylates MCM2 and MCM3 proteins in vitro, suggesting that huCdc7 may regulate processes of DNA replication by modulating MCM functions.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Cycle Proteins/chemistry,genetics,metabolism Cell Nucleus/enzymology Cells, Cultured Cloning, Molecular Cytoplasm/enzymology DNA-Binding Proteins/metabolism Gene Expression Regulation Humans Microscopy, Fluorescence Minichromosome Maintenance Complex Component 3 Molecular Sequence Data Nuclear Proteins/chemistry,metabolism Origin Recognition Complex Phosphorylation Phylogeny Protein Kinases/chemistry,genetics,metabolism Protein Serine-Threonine Kinases RNA, Messenger/metabolism Recombinant Fusion Proteins/metabolism Saccharomyces cerevisiae/enzymology Saccharomyces cerevisiae Proteins Schizosaccharomyces/enzymology Schizosaccharomyces pombe Proteins Xenopus Xenopus Proteins
Chemicals
Cell Cycle Proteins DNA-Binding Proteins MCM3 protein, human Nuclear Proteins Origin Recognition Complex RNA, Messenger Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Schizosaccharomyces pombe Proteins Xenopus Proteins Protein Kinases CDC7 protein, S cerevisiae CDC7 protein, Xenopus CDC7 protein, human cdc7 protein, S pombe Protein Serine-Threonine Kinases Minichromosome Maintenance Complex Component 3
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sato N
Department of Molecular and Developmental Biology, Institute of Medical Science, University of Tokyo, Minato-ku, Japan.
Arai K
Masai H
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/16.14.4340
Published
1997-07-16
Pages
4340-51
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1170060
Subset
IM
Databases
GENBANK
AB003698, AB003699
External Links
PubMed source DOI: 10.1093/emboj/16.14.4340 PMC Full Text View journal details
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