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PMID: 9238043 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

CCAAT/enhancer binding protein (C/EBP) sites are required for HIV-1 replication in primary macrophages but not CD4(+) T cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 94 ·No. 16 ·1997-08-05 ·Pages 8714-9

Henderson AJ, Calame KL

Abstract

The importance of CCAAT/enhancer binding proteins (C/EBPs) and binding sites for HIV-1 replication in primary macrophages, T cell lines and primary CD4(+) T cells was examined. When lines overexpressing the C/EBP dominant-negative protein LIP were infected with HIV-1, replication occurred in Jurkat T cells but not in U937 promonocytes, demonstrating a requirement for C/EBP activators by HIV-1 only in promonocytes. Primary macrophages did not support the replication of HIV-1 harboring mutant C/EBP binding sites in the long terminal repeat but Jurkat, H9 and primary CD4(+) T cells supported replication of wild-type and mutant HIV-1 equally well. Thus the requirement for C/EBP sites is also confined to monocyte/macrophages. The requirement for C/EBP proteins and sites identifies the first uniquely macrophage-specific regulatory mechanism for HIV-1 replication.

MeSH Terms
Binding Sites/immunology CCAAT-Enhancer-Binding Proteins CD4 Antigens CD4-Positive T-Lymphocytes/immunology,virology DNA-Binding Proteins/physiology HIV-1/physiology Humans Jurkat Cells Macrophages/immunology,virology Nuclear Proteins/physiology Organ Specificity Virus Replication
Chemicals
CCAAT-Enhancer-Binding Proteins CD4 Antigens DNA-Binding Proteins Nuclear Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Henderson A J
Department of Microbiology, Columbia University, College of Physicians and Surgeons, 701 West 168th Street, New York, NY 10032, USA.
Calame K L
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.94.16.8714
Published
1997-08-05
Pages
8714-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC23095
Subset
IM
Grants
NIGMS NIH HHS · GM29361 · United States
External Links
PubMed source DOI: 10.1073/pnas.94.16.8714 PMC Full Text View journal details
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