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PMID: 9211979 Published · ppublish English Journal Article

U14 small nucleolar RNA makes multiple contacts with the pre-ribosomal RNA.

Chromosoma ·Vol. 105 ·No. 7-8 ·1997-06-00 ·Pages 515-22

Morrissey JP, Tollervey D

Abstract

The small nucleolar RNA (snoRNA) U14 has two regions of extended primary sequence complementarity to the 18S rRNA. The 3' region (domain B) shows the consensus structure for the methylation guide class of snoRNAs, whereas base-pairing between the 5' region (domain A) and the 18S rRNA sequence is required for the formation of functional ribosomes. Between domains A and B lies another essential region (domain Y). Here we report that yeast U14 can be cross-linked in vivo to the pre-rRNA; cross-linking is detected exclusively with the 35S primary transcript. Many nucleotides in U14 that lie outside of domains A and B are cross-linked to the pre-rRNA; in particular the essential domain Y region is cross-linked at several sites. U14 is, therefore, in far more extensive contact with the pre-rRNA than predicted from simple base-pairing models. Moreover, U14 can be cross-linked to other small RNA species. The functional interactions made by U14 during ribosome synthesis are likely to be very complex.

MeSH Terms
Base Composition Base Sequence Binding Sites Cell Nucleolus/genetics Cross-Linking Reagents Molecular Sequence Data RNA Precursors/chemistry,genetics,metabolism RNA, Ribosomal/chemistry,genetics,metabolism RNA, Ribosomal, 18S/chemistry,genetics,metabolism RNA, Small Nuclear/chemistry,genetics,metabolism Saccharomyces cerevisiae/genetics
Chemicals
Cross-Linking Reagents RNA Precursors RNA, Ribosomal RNA, Ribosomal, 18S RNA, Small Nuclear
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Morrissey J P
EMBL, Meyerhofstrasse 1, D-69117 Heidelberg, Germany.
Tollervey D
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF02510488
Published
1997-06-00
Pages
515-22
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02510488 View journal details
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