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PMID: 9177165 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The 2.1-A crystal structure of an archaeal preinitiation complex: TATA-box-binding protein/transcription factor (II)B core/TATA-box.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 94 ·No. 12 ·1997-06-10 ·Pages 6042-7

Kosa PF, Ghosh G, DeDecker BS, Sigler PB

Abstract

Archaea possess a basal transcriptional apparatus that resembles that of eukaryotes. Here we report the 2.1-A crystal structure of the archaeal transcription factor complex formed by the TATA-box-binding protein (TBP), the transcription factor IIB homolog, and a DNA target, all from the hyperthermophile Pyrococcus woesei. The overall fold of these two basal transcription factors is essentially the same as that of their eukaryotic counterparts. However, in comparison with the eukaryotic complexes, the archaeal TBP-DNA interface is more symmetrical, and in this structure the orientation of the preinitiation complex assembly on the promoter is inverted with respect to that seen in all crystal structures of comparable eukaryotic systems. This study of the structural details of an archaeal transcription factor complex presents the opportunity to examine the evolution of the basal eukaryotic transcriptional apparatus from a stereochemical viewpoint and to extend our understanding of the physical biochemistry of transcriptional initiation.

MeSH Terms
Amino Acid Sequence Arabidopsis Archaea/metabolism Base Sequence Computer Graphics Crystallography, X-Ray DNA/chemistry,metabolism DNA-Binding Proteins/biosynthesis,chemistry,metabolism Humans Models, Molecular Molecular Sequence Data Nucleic Acid Conformation Oligodeoxyribonucleotides/chemistry,metabolism Protein Conformation Protein Folding Protein Structure, Secondary Protein Structure, Tertiary Recombinant Proteins/biosynthesis,chemistry Sequence Deletion Species Specificity TATA Box TATA-Box Binding Protein Transcription Factor TFIIB Transcription Factors/biosynthesis,chemistry,metabolism
Chemicals
DNA-Binding Proteins Oligodeoxyribonucleotides Recombinant Proteins TATA-Box Binding Protein Transcription Factor TFIIB Transcription Factors DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kosa P F
Department of Molecular Biophysics and Biochemistry, and the Howard Hughes Medical Institute, Yale University, 260 Whitney Avenue, JWG 423, New Haven CT 06511, USA.
Ghosh G
DeDecker B S
Sigler P B
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.94.12.6042
Published
1997-06-10
Pages
6042-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC20997
Subset
IM
Grants
Wellcome Trust · United Kingdom
NIGMS NIH HHS · GM15225 · United States
Databases
PDB
1AIS
External Links
PubMed source DOI: 10.1073/pnas.94.12.6042 PMC Full Text View journal details
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