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PMID: 1279520 Published · ppublish English Journal Article

Elements of an archaeal promoter defined by mutational analysis.

Nucleic acids research ·Vol. 20 ·No. 20 ·1992-10-25 ·Pages 5423-8

Hain J, Reiter WD, Hüdepohl U, Zillig W

Abstract

The sequence requirements for specific and efficient transcription from the 16S/23S rRNA promoter of Sulfolobus shibatae were analysed by point mutations and by cassette mutations using an in vitro transcription system. The examination of the box A-containing distal promoter element (DPE) showed the great importance of the TA sequence in the center of box A for transcription efficiency and the influence of the sequence upstream of box A on determining the distance between the DPE and the start site. In most positions of box A, replacement of the wild type bases by adenines or thymines are less detrimental than replacements by cytosines or guanines. The effectiveness of the proximal promoter element (PPE) was not merely determined by its high A + T content but appeared to be directly related to its nucleotide sequence. At the start site a pyrimidine/purine (py/pu) sequence was necessary for unambiguous initiation as shown by analysis of mutants where the wild type start base was replaced. The sequence of box A optimal for promoter function in vitro is identical to the consensus of 84 mapped archaeal promoter sequences.

MeSH Terms
Base Composition/genetics Base Sequence Consensus Sequence/genetics DNA Mutational Analysis Molecular Sequence Data Promoter Regions, Genetic/genetics RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics RNA, Ribosomal, 23S/genetics Sulfolobus/genetics Transcription, Genetic/genetics
Chemicals
RNA, Bacterial RNA, Ribosomal, 16S RNA, Ribosomal, 23S
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hain J
Max-Planck-Institut für Biochemie, Martinsried, Germany.
Reiter W D
Hüdepohl U
Zillig W
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/20.20.5423
Published
1992-10-25
Pages
5423-8
Language
English
Region
England
NLM ID
0411011
PMCID
PMC334351
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/20.20.5423 PMC Full Text View journal details
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