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PMID: 1729711 Published · ppublish English Comparative Study Journal Article

Component H of the DNA-dependent RNA polymerases of Archaea is homologous to a subunit shared by the three eucaryal nuclear RNA polymerases.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 1 ·1992-01-01 ·Pages 407-10

Klenk HP, Palm P, Lottspeich F, Zillig W

Abstract

The gene encoding component H of the DNA-dependent RNA polymerase (RNAP, EC 2.7.7.6) of Sulfolobus acidocaldarius has been identified by comparison of the amino acid sequence with the derived amino acid sequence of an open reading frame (ORF88) in the RNAP operon. Corresponding genes were identified in Halobacterium halobium and were cloned and sequenced from Thermococcus celer and Methanococcus vannielii. All these rpoH genes are situated between the promoters of the RNAP operons and the corresponding rpoB and rpoB2 genes. The archaeal H subunits show high sequence similarity to each other and to the C-terminal portions of the largest of four subunits shared by all three specialized nuclear RNAPs. These correlations are further evidence for the striking similarity between archaeal and eucaryal RNAP structures and transcription systems.

Related Genes
rpb5 rpoB rpoB2 rpoH
MeSH Terms
Amino Acid Sequence Archaea/enzymology,genetics Base Sequence Biological Evolution DNA-Directed RNA Polymerases/chemistry,genetics Genes, Bacterial Molecular Sequence Data Saccharomyces cerevisiae/enzymology,genetics Sequence Alignment
Chemicals
DNA-Directed RNA Polymerases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Klenk H P
Max-Planck-Institut für Biochemie, Martinsried, Federal Republic of Germany.
Palm P
Lottspeich F
Zillig W
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.1.407
Published
1992-01-01
Pages
407-10
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC48246
Subset
IM
Databases
GENBANK
X59077, X59151
External Links
PubMed source DOI: 10.1073/pnas.89.1.407 PMC Full Text View journal details
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