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PMID: 9111140 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Screening for the presence of polyglycosylceramides in various tissues: partial characterization of blood group-active complex glycosphingolipids of rabbit and dog small intestines.

Glycoconjugate journal ·Vol. 14 ·No. 2 ·1997-02-00 ·Pages 231-9

Miller-Podraza H, Stenhagen G, Larsson T, Andersson C, Karlsson KA

Abstract

Twenty different human and animal tissues were investigated for the presence of polyglycosylceramides. The glycolipids were isolated by peracetylation of dry tissue residues left after conventional lipid extraction, followed by extraction with chloroform and subsequent Sephadex LH-20, Sephadex LH-60 and silica gel chromatography. In most of the cases only trace amounts of complex glycolipids were found. Distinct bands of glycosphingolipids migrating on TLC plates in a region of brain gangliosides and below were observed in bovine erythrocytes, human leukocytes and human colon mucosa. Definite fractions of polyglycosylceramides were isolated from rabbit small intestine, dog small intestine, human placenta and human leukocytes. The polyglycosylceramides of dog and rabbit intestine were characterized by colorimetric analysis, methylation analysis, mass spectrometry and immunological assays. The dog material contained branched carbohydrate chains with repeated fucosylated N-acetyllactosamine units. Rabbit intestine polyglycosylceramides resembled rabbit erythrocyte polyglycosylceramides with Hex-Hex- terminal determinants but were more complex in respect of sugar composition and structure. The material isolated from dog intestine showed A, H, Le(x) and Le(y) blood group activities. Polyglycosylceramides of human erythrocytes, placenta and leukocytes showed strong binding affinity for Helicobacter pylori, while polyglycosylceramide fractions from rabbit and dog intestine were receptor-inactive for this bacterium or displayed only weak and poorly reproducible binding.

MeSH Terms
ABO Blood-Group System/chemistry,immunology Animals Carbohydrate Sequence Carbohydrates/analysis Cattle Ceramides/blood,chemistry,metabolism Chemical Fractionation Dogs Erythrocytes/chemistry,metabolism,microbiology Glycosphingolipids/blood,chemistry,immunology Guinea Pigs Helicobacter pylori/metabolism Humans Intestine, Small/chemistry,immunology Lewis Blood Group Antigens/chemistry,immunology Molecular Sequence Data Rabbits Rats
Chemicals
ABO Blood-Group System Carbohydrates Ceramides Glycosphingolipids Lewis Blood Group Antigens
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Miller-Podraza H
Department of Medical Biochemistry Göteborg University, Sweden.
Stenhagen G
Larsson T
Andersson C
Karlsson K A
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Article Info
Journal
Glycoconjugate journal
Abbr.
Glycoconj J
ISSN
0282-0080
DOI
10.1023/a:1018545922728
Published
1997-02-00
Pages
231-9
Language
English
Region
United States
NLM ID
8603310
Subset
IM
External Links
PubMed source DOI: 10.1023/a:1018545922728 View journal details
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