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PMID: 7531688 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation and characterization of a rat testis glycosphingolipid based on gangliotetraosylceramide and having a blood group B determinant extended with beta 3-linked N-acetylgalactosamine.

Journal of biochemistry ·Vol. 116 ·No. 3 ·1994-09-00 ·Pages 697-703

Teneberg S, Jovall PA, Karlsson H, Sjögren HO, Brodin T

Abstract

Monoclonal antibody 14.2, directed against the GalNAc beta 3Gal alpha 3Gal beta epitope, was used to monitor the isolation of a novel glycosphingolipid from rat testes. Structural analysis was performed by mass spectrometry, proton NMR spectroscopy, gas chromatography-mass spectrometry after degradation, and exoglycosidase digestion. It was concluded that the structure was [formula: see text] This is the first report on an extended blood group B determinant.

MeSH Terms

ABO Blood-Group System/immunology Acetylgalactosamine/chemistry Animals Antigen-Antibody Reactions Carbohydrate Conformation Carbohydrate Sequence Chromatography, Gas Epitopes Gangliosides Glycosphingolipids/analysis,chemistry Male Mass Spectrometry Molecular Sequence Data Rats Rats, Inbred WF Testis/chemistry

Chemicals

ABO Blood-Group System Epitopes G(A1) ganglioside Gangliosides Glycosphingolipids Acetylgalactosamine

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Teneberg S

Department of Medical Biochemistry, Göteborg University, Sweden.

Jovall P A

Karlsson H

Sjögren H O

Brodin T

Article Info

Journal

Journal of biochemistry

Abbr.

J Biochem

ISSN

0021-924X

DOI

10.1093/oxfordjournals.jbchem.a124583

Published

1994-09-00

Pages

697-703

Language

English

Region

England

NLM ID

0376600

Subset

External Links

PubMed source DOI: 10.1093/oxfordjournals.jbchem.a124583 View journal details

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Isolation and characterization of a rat testis glycosphingolipid based on gangliotetraosylceramide and having a blood group B determinant extended with beta 3-linked N-acetylgalactosamine.

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