Home LiteratureArticle Details
PMID: 9098081 Published · ppublish English Journal Article

Characterization of the genes encoding a receptor-like histidine kinase and a cognate response regulator from a biphenyl/polychlorobiphenyl-degrading bacterium, Rhodococcus sp. strain M5.

Journal of bacteriology ·Vol. 179 ·No. 8 ·1997-04-00 ·Pages 2772-6

Labbé D, Garnon J, Lau PC

Abstract

We report the cloning, sequence, and expression of the bpdS and bpdT genes from Rhodococcus sp. strain M5, which are believed to encode the first two-component signal transduction system in the genus Rhodococcus, which potentially regulates biphenyl/polychlorobiphenyl metabolism in M5. BpdT has a typical responses regulator sequence (209 amino acids; 23 kDa), whereas BpdS, the predicted histidine kinase component, is an unusually large transmembrane protein (1,576 amino acids; 170 kDa) that contains ATP-binding and leucine-rich repeat motifs and some conserved residues of protein kinases. Expression of bpdST, like that of the bpdC1C2BADE degradative operon, is inducible by biphenyl.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Biphenyl Compounds/metabolism Cloning, Molecular Gene Expression Regulation, Bacterial/genetics Genes, Bacterial/genetics Genes, Regulator/genetics Membrane Proteins Molecular Sequence Data Mutagenesis, Site-Directed Polychlorinated Biphenyls/metabolism Protein Kinases/genetics RNA, Bacterial/analysis RNA, Messenger/analysis Restriction Mapping Rhodococcus/genetics,metabolism Sequence Analysis, DNA Sequence Homology, Amino Acid Signal Transduction/genetics
Chemicals
Bacterial Proteins Biphenyl Compounds BpdT protein, Rhodococcus sp. M5 Membrane Proteins RNA, Bacterial RNA, Messenger diphenyl Polychlorinated Biphenyls Protein Kinases BpdS protein, Rhodococcus sp. M5
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Labbé D
Biotechnology Research Institute, National Research Council Canada, Montréal, Québec.
Garnon J
Lau P C
References (28)
28 references, click to expand
  1. Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold.
    EMBO J. 1982;1(8):945-51 PMID: 6329717
  2. A bacterial basic region leucine zipper histidine kinase regulating toluene degradation.
    Proc Natl Acad Sci U S A. 1997 Feb 18;94(4):1453-8 PMID: 9037074
  3. Nucleotide sequence of the uhp region of Escherichia coli.
    J Bacteriol. 1987 Aug;169(8):3556-63 PMID: 3301805
  4. The protein kinase family: conserved features and deduced phylogeny of the catalytic domains.
    Science. 1988 Jul 1;241(4861):42-52 PMID: 3291115
  5. Localization of Bacillus subtilis sacU(Hy) mutations to two linked genes with similarities to the conserved procaryotic family of two-component signalling systems.
    J Bacteriol. 1988 Nov;170(11):5102-9 PMID: 3141378
  6. Cloning and sequencing of two tandem genes involved in degradation of 2,3-dihydroxybiphenyl to benzoic acid in the polychlorinated biphenyl-degrading soil bacterium Pseudomonas sp. strain KKS102.
    J Bacteriol. 1989 May;171(5):2740-7 PMID: 2540155
  7. Protein phosphorylation and regulation of adaptive responses in bacteria.
    Microbiol Rev. 1989 Dec;53(4):450-90 PMID: 2556636
  8. A Bacillus subtilis regulatory gene product for genetic competence and sporulation resembles sensor protein members of the bacterial two-component signal-transduction systems.
    Genes Dev. 1990 May;4(5):860-72 PMID: 2116363
  9. Dual-specificity protein kinases: will any hydroxyl do?
    Trends Biochem Sci. 1992 Mar;17(3):114-9 PMID: 1412695
  10. The biology and genetics of the genus Rhodococcus.
    Annu Rev Microbiol. 1992;46:193-218 PMID: 1444254
  11. Communication modules in bacterial signaling proteins.
    Annu Rev Genet. 1992;26:71-112 PMID: 1482126
  12. Identification of protein coding regions by database similarity search.
    Nat Genet. 1993 Mar;3(3):266-72 PMID: 8485583
  13. Structural, functional, and evolutionary relationships among extracellular solute-binding receptors of bacteria.
    Microbiol Rev. 1993 Jun;57(2):320-46 PMID: 8336670
  14. A chromosomally encoded two-component sensory transduction system is required for virulence of Agrobacterium tumefaciens.
    J Bacteriol. 1993 Oct;175(20):6614-25 PMID: 8407839
  15. Structural conservation in the CheY superfamily.
    Biochemistry. 1993 Nov 9;32(44):11741-53 PMID: 8218244
  16. VsrA, a second two-component sensor regulating virulence genes of Pseudomonas solanacearum.
    Mol Microbiol. 1994 Feb;11(3):489-500 PMID: 8152373
  17. Biotransformations catalyzed by the genus Rhodococcus.
    Crit Rev Biotechnol. 1994;14(1):29-73 PMID: 8187203
  18. Protein histidine kinases and signal transduction in prokaryotes and eukaryotes.
    Trends Genet. 1994 Apr;10(4):133-8 PMID: 8029829
  19. The leucine-rich repeat: a versatile binding motif.
    Trends Biochem Sci. 1994 Oct;19(10):415-21 PMID: 7817399
  20. Molecular genetics and evolutionary relationship of PCB-degrading bacteria.
    Biodegradation. 1994 Dec;5(3-4):289-300 PMID: 7765839
  21. Response regulators of bacterial signal transduction systems: selective domain shuffling during evolution.
    J Mol Evol. 1995 Feb;40(2):136-54 PMID: 7699720
  22. The gene for the longest known Escherichia coli protein is a member of helicase superfamily II.
    J Bacteriol. 1995 Oct;177(19):5393-400 PMID: 7559321
  23. Sequence and expression of the bpdC1C2BADE genes involved in the initial steps of biphenyl/chlorobiphenyl degradation by Rhodococcus sp. M5.
    Gene. 1995 Oct 16;164(1):117-22 PMID: 7590299
  24. Location and sequence analysis of a 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase-encoding gene (bpdF) of the biphenyl/polychlorinated biphenyl degradation pathway in Rhodococcus sp. M5.
    Gene. 1996 May 24;171(1):53-7 PMID: 8675030
  25. Extraction of RNA from gram-positive bacteria.
    Biotechniques. 1995 Dec;19(6):880-84 PMID: 8747648
  26. Plant transmembrane receptors: new pieces in the signaling puzzle.
    Trends Biochem Sci. 1996 Feb;21(2):70-3 PMID: 8851664
  27. Identification and sequence analysis of two regulatory genes involved in anaerobic toluene metabolism by strain T1.
    Appl Environ Microbiol. 1997 Feb;63(2):652-60 PMID: 9023943
  28. A family of related ATP-binding subunits coupled to many distinct biological processes in bacteria.
    Nature. 1986 Oct 2-8;323(6087):448-50 PMID: 3762694
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.179.8.2772-2776.1997
Published
1997-04-00
Pages
2772-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC179032
Subset
IM
Databases
GENBANK
D13457, M24929, M34019, M80493, U85412
External Links
PubMed source DOI: 10.1128/jb.179.8.2772-2776.1997 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2