Home LiteratureArticle Details
PMID: 9098050 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The pst operon of Bacillus subtilis has a phosphate-regulated promoter and is involved in phosphate transport but not in regulation of the pho regulon.

Journal of bacteriology ·Vol. 179 ·No. 8 ·1997-04-00 ·Pages 2534-9

Qi Y, Kobayashi Y, Hulett FM

Abstract

Genes from Bacillus subtilis predicted to encode a phosphate-specific transport (Pst) system were shown by mutation to affect high-affinity Pi uptake but not arsenate resistance or phosphate (Pho) regulation. The transcription start of the promoter upstream of the pstS gene was defined by primer extension. The promoter contains structural features analogous to the Escherichia coli pst promoter but not sequence similarity. Expression from this promoter was induced >5,000-fold upon phosphate starvation and regulated by the PhoP-PhoR two-component regulatory system. These data indicate that the pst operon is involved in phosphate transport and is a member of the Pho regulon but is not involved in Pi regulation.

MeSH Terms
ATP-Binding Cassette Transporters/genetics Bacillus subtilis/genetics,metabolism Bacterial Proteins/genetics Base Sequence Biological Transport Carrier Proteins/genetics Escherichia coli Proteins Gene Expression Regulation, Bacterial/genetics Membrane Proteins/genetics Molecular Sequence Data Mutation Periplasmic Binding Proteins Phosphate-Binding Proteins Phosphates/metabolism Promoter Regions, Genetic Recombinant Fusion Proteins Regulon/genetics Sequence Homology, Nucleic Acid Transcription, Genetic/genetics
Chemicals
ATP-Binding Cassette Transporters Bacterial Proteins Carrier Proteins Escherichia coli Proteins Membrane Proteins Periplasmic Binding Proteins Phosphate-Binding Proteins Phosphates PstC protein, E coli PstC protein, bacteria PstS protein, E coli Recombinant Fusion Proteins PhoR protein, Bacteria PhoP protein, Bacteria
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Qi Y
Department of Biological Sciences, University of Illinois at Chicago, 60607, USA.
Kobayashi Y
Hulett F M
References (37)
37 references, click to expand
  1. Inorganic phosphate transport in Escherichia coli: involvement of two genes which play a role in alkaline phosphatase regulation.
    J Bacteriol. 1973 Feb;113(2):529-39 PMID: 4570598
  2. The role of polyamines in the neutralization of bacteriophage deoxyribonucleic acid.
    J Biol Chem. 1960 Mar;235:769-75 PMID: 13793161
  3. A mutant of Escherichia coli auxotrophic for organic phosphates: evidence for two defects in inorganic phosphate transport.
    Mol Gen Genet. 1975 Dec 30;143(1):71-7 PMID: 765745
  4. Two systems for the uptake of phosphate in Escherichia coli.
    J Bacteriol. 1977 Aug;131(2):505-11 PMID: 328484
  5. Transport of iron into bacterial cells.
    Methods Enzymol. 1979;56:388-94 PMID: 379514
  6. Characterization of two genetically separable inorganic phosphate transport systems in Escherichia coli.
    J Bacteriol. 1980 Oct;144(1):356-65 PMID: 6998957
  7. Effect of arsenate on inorganic phosphate transport in Escherichia coli.
    J Bacteriol. 1980 Oct;144(1):366-74 PMID: 6998959
  8. Genetic and physiological tests of three phosphate-specific transport mutants of Escherichia coli.
    J Bacteriol. 1981 Mar;145(3):1249-56 PMID: 7009575
  9. Mutants affected in alkaline phosphatase, expression: evidence for multiple positive regulators of the phosphate regulon in Escherichia coli.
    Genetics. 1980 Oct;96(2):353-66 PMID: 7021308
  10. Genetic analysis of mutants affected in the Pst inorganic phosphate transport system.
    J Bacteriol. 1981 Oct;148(1):1-9 PMID: 7026529
  11. Inorganic cation transport and the effects on C4 dicarboxylate transport in Bacillus subtilis.
    Can J Microbiol. 1981 Nov;27(11):1194-201 PMID: 6797714
  12. Cloning of and complementation tests with alkaline phosphatase regulatory genes (phoS and phoT) of Escherichia coli.
    J Bacteriol. 1982 Nov;152(2):692-701 PMID: 6290447
  13. Regulation of the phosphate regulon in Escherichia coli K-12: regulation of the negative regulatory gene phoU and identification of the gene product.
    J Bacteriol. 1984 Sep;159(3):979-85 PMID: 6090402
  14. Bacterial periplasmic transport systems: structure, mechanism, and evolution.
    Annu Rev Biochem. 1986;55:397-425 PMID: 3527048
  15. Construction of a single-copy integration vector and its use in analysis of regulation of the trp operon of Bacillus subtilis.
    Gene. 1986;43(1-2):85-94 PMID: 3019840
  16. Transcription of Bacillus subtilis subtilisin and expression of subtilisin in sporulation mutants.
    J Bacteriol. 1988 Jan;170(1):289-95 PMID: 2447062
  17. Regulation of the phosphate regulon of Escherichia coli. Activation of pstS transcription by PhoB protein in vitro.
    J Mol Biol. 1988 Sep 5;203(1):85-95 PMID: 3054125
  18. Regulation of the phosphate regulon of Escherichia coli: characterization of the promoter of the pstS gene.
    Mol Gen Genet. 1989 Feb;215(3):374-80 PMID: 2651888
  19. Evidence for two structural genes for alkaline phosphatase in Bacillus subtilis.
    J Bacteriol. 1990 Feb;172(2):735-40 PMID: 2105301
  20. Signal transduction pathway controlling synthesis of a class of degradative enzymes in Bacillus subtilis: expression of the regulatory genes and analysis of mutations in degS and degU.
    J Bacteriol. 1990 Feb;172(2):824-34 PMID: 1688843
  21. Signal transduction in the phosphate regulon of Escherichia coli involves phosphotransfer between PhoR and PhoB proteins.
    J Mol Biol. 1989 Dec 5;210(3):551-9 PMID: 2693738
  22. From cell membrane to nucleotides: the phosphate regulon in Escherichia coli.
    Bioessays. 1990 Aug;12(8):371-6 PMID: 2241934
  23. Separate promoters direct expression of phoAIII, a member of the Bacillus subtilis alkaline phosphatase multigene family, during phosphate starvation and sporulation.
    Mol Microbiol. 1991 Sep;5(9):2181-90 PMID: 1766385
  24. Mutational analysis of the Escherichia coli phosphate-specific transport system, a member of the traffic ATPase (or ABC) family of membrane transporters. A role for proline residues in transmembrane helices.
    J Biol Chem. 1992 Dec 5;267(34):24661-8 PMID: 1447208
  25. Gene regulation by phosphate in enteric bacteria.
    J Cell Biochem. 1993 Jan;51(1):47-54 PMID: 8432742
  26. Use of the rep technique for allele replacement to construct mutants with deletions of the pstSCAB-phoU operon: evidence of a new role for the PhoU protein in the phosphate regulon.
    J Bacteriol. 1993 Nov;175(21):6797-809 PMID: 8226621
  27. Translocation of metal phosphate via the phosphate inorganic transport system of Escherichia coli.
    Biochemistry. 1994 Feb 22;33(7):1766-70 PMID: 8110778
  28. Sequential action of two-component genetic switches regulates the PHO regulon in Bacillus subtilis.
    J Bacteriol. 1994 Mar;176(5):1348-58 PMID: 8113174
  29. A pho regulon promoter induced under sporulation conditions.
    Gene. 1994 Sep 15;147(1):95-100 PMID: 8088554
  30. The sigma factors of Bacillus subtilis.
    Microbiol Rev. 1995 Mar;59(1):1-30 PMID: 7708009
  31. Modulation of a salt link does not affect binding of phosphate to its specific active transport receptor.
    Biochemistry. 1996 Feb 20;35(7):2079-85 PMID: 8652549
  32. Molecular analysis of the phosphate-specific transport (pst) operon of Pseudomonas aeruginosa.
    Mol Gen Genet. 1996 Apr 10;250(6):692-8 PMID: 8628229
  33. The signal-transduction network for Pho regulation in Bacillus subtilis.
    Mol Microbiol. 1996 Mar;19(5):933-9 PMID: 8830274
  34. Negative electrostatic surface potential of protein sites specific for anionic ligands.
    Proc Natl Acad Sci U S A. 1996 Jun 25;93(13):6786-91 PMID: 8692896
  35. A Bacillus subtilis gene cluster similar to the Escherichia coli phosphate-specific transport (pst) operon: evidence for a tandemly arranged pstB gene.
    Microbiology. 1996 Aug;142 ( Pt 8):2017-20 PMID: 8760913
  36. A Bacillus subtilis secreted phosphodiesterase/alkaline phosphatase is the product of a Pho regulon gene, phoD.
    Microbiology. 1996 Aug;142 ( Pt 8):2041-7 PMID: 8760916
  37. Succinate transport in Bacillus subtilis. Dependence on inorganic anions.
    Biochim Biophys Acta. 1975 Sep 2;401(3):440-57 PMID: 810162
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.179.8.2534-2539.1997
Published
1997-04-00
Pages
2534-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC179001
Subset
IM
Databases
GENBANK
D58414
External Links
PubMed source DOI: 10.1128/jb.179.8.2534-2539.1997 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2