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PMID: 765745 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A mutant of Escherichia coli auxotrophic for organic phosphates: evidence for two defects in inorganic phosphate transport.

Molecular & general genetics : MGG ·Vol. 143 ·No. 1 ·1975-12-30 ·Pages 71-7

Sprague GF, Bell RM, Cronan JE

Abstract

An inorganic phosphate transport mutant has been isolated as a sn-glycerol-3-phosphate auxotroph and characterized genetically. Two lesions are responsible for the transport defect. One lesion, pst, is located at minute 74 of the E. coli genetic map while the other lesion, pit, is located at minute 68. All "K10" strains that were examined carry the pit lesion. Evidence is presented that the glycerol phosphate and hexose phosphate transport systems are not important inorganic phophate transport systems. The mapping data indicate that the genetic distance between malA and xyl is greater than that now allowed.

MeSH Terms
Bacterial Proteins/biosynthesis Biological Transport, Active Chromosome Mapping Chromosomes, Bacterial Coliphages Conjugation, Genetic DNA, Bacterial/biosynthesis Escherichia coli/metabolism Genes Glycerol-3-Phosphate O-Acyltransferase/metabolism Glycerolphosphate Dehydrogenase/metabolism Glycerophosphates/metabolism Mutation Phenotype Phosphates/metabolism Phospholipids/biosynthesis Phosphotransferases/metabolism RNA, Bacterial/biosynthesis Recombination, Genetic Transduction, Genetic
Chemicals
Bacterial Proteins DNA, Bacterial Glycerophosphates Phosphates Phospholipids RNA, Bacterial Glycerolphosphate Dehydrogenase Glycerol-3-Phosphate O-Acyltransferase Phosphotransferases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sprague G F
Bell R M
Cronan J E
References (19)
19 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00269422
Published
1975-12-30
Pages
71-7
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00269422
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