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PMID: 9094637 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Negative effects of chemical mutagenesis on the adaptive behavior of vesicular stomatitis virus.

Journal of virology ·Vol. 71 ·No. 5 ·1997-05-00 ·Pages 3636-40

Lee CH, Gilbertson DL, Novella IS, Huerta R, Domingo E, Holland JJ

Abstract

Changes in adaptability of vesicular stomatitis virus (VSV) upon treatment with chemical mutagens have been investigated. Results showed no improvement in virus viability or adaptability at any given level of mutagenesis. In fact, increasing inhibition of virus production and adaptability was observed with increasing levels of mutagenesis. This was true for all tested VSV variants replicating either in changing or constant host cell environments. Results also showed that mutagen-treated RNA virus populations which had undergone severe fitness declines were able to recover lost fitness completely after several large-population passages in BHK21, cells. The present findings illustrate the highly optimized states of RNA viruses and their potential to adapt readily. These results are significant for the possible development of specific antiviral agents designed to be mutagenic.

MeSH Terms

Adaptation, Physiological Animals Cell Line Cricetinae Mutagenesis Vesicular stomatitis Indiana virus/drug effects,physiology

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Lee C H

Department of Biology and Institute of Molecular Genetics, University of California, San Diego, La Jolla 92093, USA.

Gilbertson D L

Novella I S

Huerta R

Domingo E

Holland J J

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.71.5.3636-3640.1997

Published

1997-05-00

Pages

3636-40

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC191512

Subset

Grants

NIAID NIH HHS · 5R37 AI4627-29 · United States

External Links

PubMed source DOI: 10.1128/JVI.71.5.3636-3640.1997 PMC Full Text View journal details

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Negative effects of chemical mutagenesis on the adaptive behavior of vesicular stomatitis virus.

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