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PMID: 9050864 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

GTP-bound forms of rab6 induce the redistribution of Golgi proteins into the endoplasmic reticulum.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 94 ·No. 5 ·1997-03-04 ·Pages 1828-33

Martinez O, Antony C, Pehau-Arnaudet G, Berger EG, Salamero J, Goud B

Abstract

rab6 is a ubiquitous ras-like GTPase involved in intra-Golgi transport. We have studied at both morphological and biochemical levels the behavior of Golgi resident proteins in HeLa cells overexpressing wild-type rab6 and GTP- and GDP-bound mutants of rab6 (rab6 Q72L and rab6 T27N, respectively). We show that wild-type rab6 and rab6 Q72L overexpression induces the redistribution of the trans-Golgi protein beta-1,4-galactosyltransferase into the endoplasmic reticulum (ER) and allows the addition of sialylated O-glycans on an ER-retained protein, the major histocompatibility complex class II-associated invariant chain. Remarkably, rab6 Q72L effects, which require the integrity of microtubules, were almost indistinguishable from those induced by brefeldin A, a fungic metabolite that causes a mixing of Golgi and ER membranes. In contrast, overexpression of rab6 T27N does not cause the redistribution of Golgi proteins, but inhibits basal O-glycosylation of the major histocompatibility complex class II-associated invariant chain.

MeSH Terms
Amino Acid Sequence Antigens, Differentiation, B-Lymphocyte/metabolism Biomarkers Brefeldin A Carrier Proteins/genetics,metabolism Cyclopentanes/pharmacology Endoplasmic Reticulum/metabolism Fluorescent Antibody Technique Gene Expression Regulation/genetics Glycosylation Golgi Apparatus/enzymology,metabolism Guanosine Diphosphate/metabolism Guanosine Triphosphate/metabolism HeLa Cells Histocompatibility Antigens Class II/metabolism Humans Lectins/metabolism Microtubules/metabolism Molecular Sequence Data N-Acetyllactosamine Synthase/metabolism Plant Lectins Transfection/genetics rab GTP-Binding Proteins ras Proteins/genetics,metabolism
Chemicals
Antigens, Differentiation, B-Lymphocyte Biomarkers Carrier Proteins Cyclopentanes Histocompatibility Antigens Class II Lectins Plant Lectins Rab6 protein invariant chain jacalin Guanosine Diphosphate Brefeldin A Guanosine Triphosphate N-Acetyllactosamine Synthase rab GTP-Binding Proteins ras Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Martinez O
Unité Mixte de Recherche, Institut Curie/Centre National de la Recherche Scientifique 144, Paris, France.
Antony C
Pehau-Arnaudet G
Berger E G
Salamero J
Goud B
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.94.5.1828
Published
1997-03-04
Pages
1828-33
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC20002
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.94.5.1828 PMC Full Text View journal details
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