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PMID: 904030 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Phosphorylated protein component present in influenza virions.

Journal of virology ·Vol. 24 ·No. 1 ·1977-10-00 ·Pages 401-5

Privalsky ML, Penhoet EE

Abstract

The nucleoprotein of the WSN strain of influenza was found to be phosphorylated in vitro. The phosphate-protein bond was stable to hot trichloroacetic acid, RNase, DNase, succinic acid, and succinic acid-hydroxylamine, but sensitive to hydrolysis by bacterial alkaline phosphatase. This suggested that the nucleoprotein is in the form of a phosphomonoester. Acid hydrolysis of the isolated nucleoprotein followed by thin-layer electrophoresis identified the phosphorylated amino acid residue as phosphoserine.

MeSH Terms
Alkaline Phosphatase/metabolism Hydrochloric Acid Hydrolysis Orthomyxoviridae/analysis Phosphoproteins/analysis RNA, Viral/analysis Serine/analysis Viral Proteins/analysis
Chemicals
Phosphoproteins RNA, Viral Viral Proteins Serine Alkaline Phosphatase Hydrochloric Acid
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Privalsky M L
Penhoet E E
References (14)
14 references, click to expand
  1. A study of the substrate specificity and other properties of the alkaline phosphatase of Escherichia coli.
    J Biol Chem. 1962 Mar;237:841-6 PMID: 13906598
  2. Transcription of the influenza ribonucleic acid genome by a virion polymerase. 3. Completeness of the transcription process.
    J Virol. 1972 Oct;10(4):689-97 PMID: 5084464
  3. Protein kinase and phosphate acceptor proteins in Rauscher murine leukaemia virus.
    Nat New Biol. 1971 Sep 29;233(39):137-40 PMID: 4330074
  4. Phosphoproteins, structural components of rhabdoviruses.
    Virology. 1973 Mar;52(1):246-63 PMID: 4372781
  5. Protein kinase associated with RNA tumor viruses and other budding RNA viruses.
    Virology. 1972 Feb;47(2):536-8 PMID: 4333745
  6. Protein kinase in enveloped herpes simplex virions.
    Virology. 1972 Oct;50(1):287-90 PMID: 4343113
  7. Structural proteins of simian virus 40: phosphoproteins.
    J Virol. 1972 Nov;10(5):985-94 PMID: 4344258
  8. Phosphorylation of animal virus proteins by a virion protein kinase.
    J Virol. 1973 Sep;12(3):511-22 PMID: 4355852
  9. Phosphorylation of distinct regions of f1 histone. Relationship to the cell cycle.
    J Biol Chem. 1976 Jun 25;251(12):3685-92 PMID: 180006
  10. Endogenous protein kinase and phosphate acceptor proteins in vaccinia virus.
    Virology. 1973 Mar;52(1):13-21 PMID: 4803391
  11. Phosphoprotein component of vaccinia virions.
    J Virol. 1973 Jun;11(6):961-70 PMID: 4712965
  12. Phosphorylated polypeptides in adenovirus-infected cells.
    Virology. 1972 Dec;50(3):931-4 PMID: 4640692
  13. Protein kinase and specific phosphate acceptor proteins associated with vaccinia virus cores.
    J Virol. 1972 Sep;10(3):417-24 PMID: 5072434
  14. Message activity of influenza viral RNA.
    J Virol. 1976 May;18(2):812-6 PMID: 1271526
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.24.1.401-405.1977
Published
1977-10-00
Pages
401-5
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC515941
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.24.1.401-405.1977 PMC Full Text View journal details
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