Home LiteratureArticle Details
PMID: 1271526 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Message activity of influenza viral RNA.

Journal of virology ·Vol. 18 ·No. 2 ·1976-05-00 ·Pages 812-6

Tekamp P, Penhoet EE

Abstract

The message activity of influenza virion RNA in the wheat germ cell-free protein-synthesizing system was investigated. RNA extracted from purified virions was found to direct the synthesis of a polypeptide that had the mobility of viral nucleocapsid protein on sodium dodecyl sulfate-polyacrylamide gels. Further characterization of the protein indicated it was not the nucleocapsid protein. No other polypeptides were detected. We conclude that influenza virion RNA is inactive as a template for the synthesis of virus-specific proteins.

MeSH Terms
Amino Acids/metabolism Cell-Free System Orthomyxoviridae/metabolism Peptide Biosynthesis Plant Extracts Protein Biosynthesis RNA, Messenger/metabolism RNA, Viral/metabolism Templates, Genetic Triticum Viral Proteins/biosynthesis
Chemicals
Amino Acids Plant Extracts RNA, Messenger RNA, Viral Viral Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tekamp P
Penhoet E E
References (24)
24 references, click to expand
  1. Translation of reovirus mRNA, poliovirus RNA and bacteriophage Qbeta RNA in cell-free extracts of mammalian cells.
    Methods Enzymol. 1974;30:709-23 PMID: 4369395
  2. Studies on the replication of influenza virus RNA.
    Virology. 1972 Mar;47(3):823-32 PMID: 4622296
  3. Isolation of eukaryotic messenger RNA on cellulose and its translation in vitro.
    Biochem Biophys Res Commun. 1972 Nov 1;49(3):680-9 PMID: 4638747
  4. Influenza virus messenger ribonucleoprotein.
    Virology. 1975 Sep;67(1):209-18 PMID: 1162904
  5. Cell-free transfer of leucine by transfer ribonucleic acid from mouse liver and plasma cell tumors into rabbit hemoglobin.
    Biochemistry. 1970 Feb 3;9(3):489-95 PMID: 4190247
  6. RNA-dependent RNA polymerase activity associated with virions and subviral particles of myxoviruses.
    Proc Natl Acad Sci U S A. 1971 Apr;68(4):752-6 PMID: 4325103
  7. Direct evidence for messenger activity of influenza virion RNA.
    Proc Natl Acad Sci U S A. 1973 Oct;70(10):2960-3 PMID: 4355376
  8. Cell-free hemoglobin synthesis. II. Characteristics of the transfer ribonucleic acid-dependent assay system.
    J Biol Chem. 1970 May 10;245(9):2342-9 PMID: 4909890
  9. Transcription of the influenza ribonucleic acid genome by a virion polymerase. 3. Completeness of the transcription process.
    J Virol. 1972 Oct;10(4):689-97 PMID: 5084464
  10. Isolation of influenza virus ribonucleoprotein from infected cells. Demonstration of the presence of negative-stranded RNA in viral RNP.
    Virology. 1971 Oct;46(1):149-60 PMID: 5166352
  11. Isolation of the nucleic acid of Newcastle disease virus (NDV).
    Proc Natl Acad Sci U S A. 1965 Sep;54(3):794-800 PMID: 5217460
  12. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  13. ENZYMATIC SYNTHESIS OF RIBONUCLEIC ACID. IV. THE DEOXYRIBONUCLEIC ACID-DIRECTED SYNTHESIS OF COMPLEMENTARY CYTOPLASMIC RIBONUCLEIC ACID COMPONENTS.
    J Biol Chem. 1964 Sep;239:2944-51 PMID: 14217880
  14. A DEMONSTRATION OF CODING DEGENERACY FOR LEUCINE IN THE SYNTHESIS OF PROTEIN.
    Proc Natl Acad Sci U S A. 1965 Feb;53:328-34 PMID: 14294065
  15. Synthesis of all the gene products of the reovirus genome in vivo and in vitro.
    Cell. 1975 Feb;4(2):173-80 PMID: 1125978
  16. Purification of influenza viral complementary RNA: its genetic content and activity in wheat germ cell-free extracts.
    J Virol. 1975 Dec;16(6):1464-75 PMID: 1202245
  17. Cell-free translation of influenza virus messenger RNA.
    Virology. 1973 Dec;56(2):654-7 PMID: 4202176
  18. Cell-free translation of messenger RNA of simian virus 40: synthesis of the major capsid protein.
    Proc Natl Acad Sci U S A. 1974 Feb;71(2):302-6 PMID: 4360938
  19. Influenza viral messenger RNA.
    Virology. 1974 Nov;62(1):38-45 PMID: 4473156
  20. Isolation of rabbit reticulocyte 9 S mRNA.
    Methods Enzymol. 1974;30:613-21 PMID: 4852590
  21. A procedure for the isolation of mammalian messenger ribonucleic acid.
    Biochemistry. 1972 Feb 15;11(4):637-41 PMID: 5011971
  22. Binding of ribonucleic acids to the RNP-antigen protein of influenza viruses.
    J Gen Virol. 1971 Jan;10(1):11-6 PMID: 5100698
  23. RNA-dependent RNA polymerase activity in influenza virions.
    Proc Natl Acad Sci U S A. 1971 Jun;68(6):1369-71 PMID: 5288388
  24. Characterization of a highly efficient protein synthesizing system derived from commercial wheat germ.
    Nucleic Acids Res. 1974 Nov;1(11):1385-97 PMID: 10793698
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.18.2.812-816.1976
Published
1976-05-00
Pages
812-6
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC515614
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.18.2.812-816.1976 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2