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PMID: 9037087 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification of PN1, a predominant voltage-dependent sodium channel expressed principally in peripheral neurons.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 94 ·No. 4 ·1997-02-18 ·Pages 1527-32

Toledo-Aral JJ, Moss BL, He ZJ, Koszowski AG, Whisenand T, Levinson SR, Wolf JJ, Silos-Santiago I, Halegoua S, Mandel G

Abstract

Membrane excitability in different tissues is due, in large part, to the selective expression of distinct genes encoding the voltage-dependent sodium channel. Although the predominant sodium channels in brain, skeletal muscle, and cardiac muscle have been identified, the major sodium channel types responsible for excitability within the peripheral nervous system have remained elusive. We now describe the deduced primary structure of a sodium channel, peripheral nerve type 1 (PN1), which is expressed at high levels throughout the peripheral nervous system and is targeted to nerve terminals of cultured dorsal root ganglion neurons. Studies using cultured PC12 cells indicate that both expression and targeting of PN1 is induced by treatment of the cells with nerve growth factor. The preferential localization suggests that the PN1 sodium channel plays a specific role in nerve excitability.

MeSH Terms
Amino Acid Sequence Animals Cell Compartmentation DNA, Complementary/genetics Ganglia, Spinal/chemistry,cytology Gene Expression Gene Library Immunohistochemistry In Situ Hybridization Molecular Sequence Data NAV1.7 Voltage-Gated Sodium Channel Neurites/chemistry Neurons/chemistry Neuropeptides/classification,genetics PC12 Cells Peripheral Nervous System/chemistry,cytology Polymerase Chain Reaction Rats Sodium Channels/classification,genetics Synapses/chemistry Tissue Distribution
Chemicals
DNA, Complementary NAV1.7 Voltage-Gated Sodium Channel Neuropeptides Scn9a protein, rat Sodium Channels
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Toledo-Aral J J
Department of Neurobiology and Behavior, State University of New York at Stony Brook, 11794-5230, USA.
Moss B L
He Z J
Koszowski A G
Whisenand T
Levinson S R
Wolf J J
Silos-Santiago I
Halegoua S
Mandel G
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.94.4.1527
Published
1997-02-18
Pages
1527-32
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC19825
Subset
IM
Grants
NINDS NIH HHS · NS18218 · United States
NINDS NIH HHS · R01 NS018218 · United States
NINDS NIH HHS · NS22518 · United States
NINDS NIH HHS · NS34375 · United States
NINDS NIH HHS · R01 NS022518 · United States
Databases
GENBANK
U79568
External Links
PubMed source DOI: 10.1073/pnas.94.4.1527 PMC Full Text View journal details
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