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PMID: 8394370 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Neuronal growth factor regulation of two different sodium channel types through distinct signal transduction pathways.

The Journal of cell biology ·Vol. 122 ·No. 4 ·1993-08-00 ·Pages 915-21

D'Arcangelo G, Paradiso K, Shepherd D, Brehm P, Halegoua S, Mandel G

Abstract

Neuronal growth factors regulate the expression of voltage-activated sodium current in differentiating sympathetic neurons and PC12 cells. We show that, in PC12 cells, the NGF- and FGF-induced sodium current results from increased expression of two distinct sodium channel types. Sodium current results from the rapid induction of a novel sodium channel transcript, also found in peripheral neurons, and from the long term induction of brain type II/IIA mRNA. Expression of the type II/IIA sodium channel requires activation of the cyclic AMP-dependent protein kinase (A-kinase), whereas induction of the peripheral neuron type sodium channel occurs through an A-kinase-independent signal transduction pathway. These findings suggest that the two sodium channel types act in concert to ensure the generation of action potentials during neuronal differentiation.

MeSH Terms
Animals Cyclic AMP/physiology Fibroblast Growth Factors/pharmacology Gene Expression In Vitro Techniques Membrane Potentials Nerve Growth Factors/physiology PC12 Cells Protein Kinases/metabolism RNA, Messenger/genetics Rats Signal Transduction Sodium/physiology Sodium Channels/physiology Time Factors Transcription, Genetic
Chemicals
Nerve Growth Factors RNA, Messenger Sodium Channels Fibroblast Growth Factors Sodium Cyclic AMP Protein Kinases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
D'Arcangelo G
Department of Neurobiology and Behavior, State University of New York, Stony Brook 11794-5230.
Paradiso K
Shepherd D
Brehm P
Halegoua S
Mandel G
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46 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.122.4.915
Published
1993-08-00
Pages
915-21
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2119579
Subset
IM
Grants
NINDS NIH HHS · NS 18218 · United States
NINDS NIH HHS · NS 22518 · United States
External Links
PubMed source DOI: 10.1083/jcb.122.4.915 PMC Full Text View journal details
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