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PMID: 9017597 Published · ppublish English Journal Article

Interaction of a Dictyostelium member of the plastin/fimbrin family with actin filaments and actin-myosin complexes.

Molecular biology of the cell ·Vol. 8 ·No. 1 ·1997-01-00 ·Pages 83-95

Prassler J, Stocker S, Marriott G, Heidecker M, Kellermann J, Gerisch G

Abstract

A protein purified from cytoskeletal fractions of Dictyostelium discoideum proved to be a member of the fimbrin/plastin family of actin-bundling proteins. Like other family members, this Ca(2+)-inhibited 67-kDa protein contains two EF hands followed by two actin-binding sites of the alpha-actinin/beta-spectrin type. Dd plastin interacted selectively with actin isoforms: it bound to D. discoideum actin and to beta/gamma-actin from bovine spleen but not to alpha-actin from rabbit skeletal muscle. Immunofluorescence labeling of growth phase cells showed accumulation of Dd plastin in cortical structures associated with cell surface extensions. In the elongated, streaming cells of the early aggregation stage, Dd plastin was enriched in the front regions. To examine how the bundled actin filaments behave in myosin II-driven motility, complexes of F-actin and Dd plastin were bound to immobilized heavy meromyosin, and motility was started by photoactivating caged ATP. Actin filaments were immediately propelled out of bundles or even larger aggregates and moved on the myosin as separate filaments. This result shows that myosin can disperse an actin network when it acts as a motor and sheds light on the dynamics of protein-protein interactions in the cortex of a motile cell where myosin II and Dd plastin are simultaneously present.

MeSH Terms
Actins/metabolism,ultrastructure Amino Acid Sequence Animals Binding Sites Calcium/metabolism,pharmacology Cattle Cross-Linking Reagents Dictyostelium/chemistry,growth & development,ultrastructure Membrane Glycoproteins/chemistry,metabolism Microfilament Proteins Molecular Sequence Data Muscle, Skeletal/chemistry Myosins/metabolism,ultrastructure Phosphoproteins/chemistry,genetics,metabolism Rabbits Sequence Homology, Amino Acid Spleen/chemistry
Chemicals
Actins Cross-Linking Reagents Membrane Glycoproteins Microfilament Proteins Phosphoproteins plastin Myosins Calcium
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Prassler J
Max-Planck-Institut für Biochemie, Martinsried, Germany.
Stocker S
Marriott G
Heidecker M
Kellermann J
Gerisch G
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.8.1.83
Published
1997-01-00
Pages
83-95
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC276061
Subset
IM
Databases
GENBANK
A35836, A37097, L36202, S31765
External Links
PubMed source DOI: 10.1091/mbc.8.1.83 PMC Full Text View journal details
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