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PMID: 8990291 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequences and regulational analysis of genes involved in conversion of aniline to catechol in Pseudomonas putida UCC22(pTDN1).

Journal of bacteriology ·Vol. 179 ·No. 2 ·1997-01-00 ·Pages 399-408

Fukumori F, Saint CP

Abstract

A 9,233-bp HindIII fragment of the aromatic amine catabolic plasmid pTDN1, isolated from a derivative of Pseudomonas putida mt-2 (UCC22), confers the ability to degrade aniline on P. putida KT2442. The fragment encodes six open reading frames which are arranged in the same direction. Their 5' upstream region is part of the direct-repeat sequence of pTDN1. Nucleotide sequence of 1.8 kb of the repeat sequence revealed only a single base pair change compared to the known sequence of IS1071 which is involved in the transposition of the chlorobenzoate genes (C. Nakatsu, J. Ng, R. Singh, N. Straus, and C. Wyndham, Proc. Natl. Acad. Sci. USA 88:8312-8316, 1991). Four open reading frames encode proteins with considerable homology to proteins found in other aromatic-compound degradation pathways. On the basis of sequence similarity, these genes are proposed to encode the large and small subunits of aniline oxygenase (tdnA1 and tdnA2, respectively), a reductase (tdnB), and a LysR-type regulatory gene (tdnR). The putative large subunit has a conserved [2Fe-2S]R Rieske-type ligand center. Two genes, tdnQ and tdnT, which may be involved in amino group transfer, are localized upstream of the putative oxygenase genes. The tdnQ gene product shares about 30% similarity with glutamine synthetases; however, a pUC-based plasmid carrying tdnQ did not support the growth of an Escherichia coli glnA strain in the absence of glutamine. TdnT possesses domains that are conserved among amidotransferases. The tdnQ, tdnA1, tdnA2, tdnB, and tdnR genes are essential for the conversion of aniline to catechol.

MeSH Terms
Amino Acid Sequence Aniline Compounds/metabolism Bacterial Proteins/genetics Base Sequence Catechols/metabolism DNA, Bacterial Gene Expression Regulation, Bacterial Genes, Bacterial Genetic Complementation Test Molecular Sequence Data Oxidoreductases/genetics Pseudomonas putida/genetics,metabolism Sequence Homology, Amino Acid
Chemicals
Aniline Compounds Bacterial Proteins Catechols DNA, Bacterial TdnR protein, Pseudomonas putida Oxidoreductases TdnB protein, Pseudomonas putida catechol aniline
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fukumori F
Department of Microbiology, Monash University, Clayton, Victoria, Australia. fukumori@mstunx.jamstec.go.jp
Saint C P
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.179.2.399-408.1997
Email
fukumori@mstunx.jamstec.go.jp
Published
1997-01-00
Pages
399-408
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC178709
Subset
IM
Databases
GENBANK
D85415
External Links
PubMed source DOI: 10.1128/jb.179.2.399-408.1997 PMC Full Text View journal details
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