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PMID: 1503750 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A general, PCR-based method for single or combinatorial oligonucleotide-directed mutagenesis on pUC/M13 vectors.

BioTechniques ·Vol. 12 ·No. 4 ·1992-04-00 ·Pages 508-10

Merino E, Osuna J, Bolívar F, Soberón X

Abstract

暂无摘要

MeSH Terms
Base Sequence Escherichia coli Molecular Sequence Data Mutagenesis, Site-Directed Oligonucleotides/genetics Plasmids Polymerase Chain Reaction/methods Transformation, Genetic
Chemicals
Oligonucleotides
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Merino E
Instituto de Biotecnología, UNAM, Cuernavaca, Morelos, Mexico.
Osuna J
Bolívar F
Soberón X
Article Info
Journal
BioTechniques
Abbr.
Biotechniques
ISSN
0736-6205
Published
1992-04-00
Pages
508-10
Language
English
Region
England
NLM ID
8306785
Subset
IM
External Links
PubMed source View journal details
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