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PMID: 8987740 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

A direct comparison of the single-channel properties of synaptic and extrasynaptic NMDA receptors.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 17 ·No. 1 ·1997-01-01 ·Pages 107-16

Clark BA, Farrant M, Cull-Candy SG

Abstract

The assumption that synaptic and extrasynaptic glutamate receptors are similar underpins many studies that have sought to relate the behavior of channels in excised patches to the macroscopic properties of the EPSC. We have examined this issue for NMDA receptors in cerebellar granule cells, the small size of which allows the opening of individual synaptic NMDA channels to be resolved directly. We have used whole-cell patch-clamp recordings to determine the conductance and open time of NMDA channels activated during the EPSC and used cell-attached and outside-out recordings to examine NMDA receptors in somatic membrane. Conductance and open time of synaptic channels were indistinguishable from those of extrasynaptic channels in cell-attached patches. However, the channel conductance in outside-out patches was 20% lower than in cell-attached recordings. This change was partially reduced by dantrolene and phalloidin, suggesting that it may involve depolymerization of actin following Ca2+ release from intracellular stores. Our results demonstrate that synaptic and extrasynaptic NMDA receptors have similar microscopic properties. However, NMDA channel conductance is reduced following the formation of an outside-out patch.

MeSH Terms
Animals Cerebellum/cytology,metabolism Electric Conductivity Ion Channels/physiology Patch-Clamp Techniques Rats Rats, Sprague-Dawley Receptors, N-Methyl-D-Aspartate/metabolism Synapses/physiology
Chemicals
Ion Channels Receptors, N-Methyl-D-Aspartate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Clark B A
Department of Pharmacology, University College London, London WC1E 6BT, United Kingdom.
Farrant M
Cull-Candy S G
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
0270-6474
Published
1997-01-01
Pages
107-16
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6793703
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source PMC Full Text View journal details
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