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PMID: 8986806 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A novel member of the RING finger family, KRIP-1, associates with the KRAB-A transcriptional repressor domain of zinc finger proteins.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 93 ·No. 26 ·1996-12-24 ·Pages 15299-304

Kim SS, Chen YM, O'Leary E, Witzgall R, Vidal M, Bonventre JV

Abstract

The Krüppel-associated box A (KRAB-A) domain is an evolutionarily conserved transcriptional repressor domain present in approximately one-third of zinc finger proteins of the Cys2-His2 type. Using the yeast two-hybrid system, we report the isolation of a cDNA encoding a novel murine protein, KRAB-A interacting protein 1 (KRIP-1) that physically interacts with the KRAB-A region. KRIP-1 is a member of the RBCC subfamily of the RING finger, or Cys3HisCys4, family of zinc binding proteins whose other members are known to play important roles in differentiation, oncogenesis, and signal transduction. The KRIP-1 protein has high homology to TIF1, a putative modulator of ligand-dependent activation function of nuclear receptors. A 3.5-kb mRNA for KRIP-1 is ubiquitously expressed among all adult mouse tissues studied. When a GAL4-KRIP-1 fusion protein is expressed in COS cells with a chloramphenicol acetyltransferase reporter construct with five GAL4 binding sites, there is dose-dependent repression of transcription. Thus, KRIP-1 interacts with the KRAB-A region of C2H2 zinc finger proteins and may mediate or modulate KRAB-A transcriptional repressor activity.

MeSH Terms

Amino Acid Sequence Animals Base Sequence COS Cells Conserved Sequence Embryo, Mammalian Gene Library Kidney/metabolism Mice Molecular Sequence Data Nuclear Proteins/biosynthesis,chemistry,metabolism Recombinant Fusion Proteins/metabolism Repressor Proteins/metabolism Transcription Factors/biosynthesis,chemistry,metabolism Transfection Tripartite Motif-Containing Protein 28 Zinc Fingers

Chemicals

Nuclear Proteins Recombinant Fusion Proteins Repressor Proteins Transcription Factors Trim28 protein, mouse Tripartite Motif-Containing Protein 28

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Kim S S

Renal Unit, Massachusetts General Hospital, Charlestown 02129, USA.

Chen Y M

O'Leary E

Witzgall R

Vidal M

Bonventre J V

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.93.26.15299

Published

1996-12-24

Pages

15299-304

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC26399

Subset

Grants

NINDS NIH HHS · P01 NS010828 · United States

NIDDK NIH HHS · R37 DK039773 · United States

NIDDK NIH HHS · DK 39773 · United States

NINDS NIH HHS · P50 NS010828 · United States

NINDS NIH HHS · F32 NS010828 · United States

NIDDK NIH HHS · DK 38452 · United States

NIDDK NIH HHS · P01 DK038452 · United States

NIDDK NIH HHS · R01 DK039773 · United States

NINDS NIH HHS · NS 10828 · United States

Databases

GENBANK

U67303

External Links

PubMed source DOI: 10.1073/pnas.93.26.15299 PMC Full Text View journal details

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A novel member of the RING finger family, KRIP-1, associates with the KRAB-A transcriptional repressor domain of zinc finger proteins.

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