Abstract
We have previously reported the cloning, sequencing, and partial characterization of Kid-1, a zinc finger-encoding cDNA from the rat kidney. The Kid-1 protein and approximately one-third of all other zinc finger proteins contain a highly conserved region of approximately 75 amino acids at their NH2 terminus named Krüppel-associated box (KRAB), which is subdivided into A and B domains. The evolutionary conservation, wide distribution, and genomic organization of the KRAB domains suggest an important role of this region in the transcriptional regulatory function of zinc finger proteins. The functional significance of the KRAB domain was evaluated by studying transcriptional activities of yeast GAL4-rat Kid-1 fusion proteins containing various regions of the non-zinc-finger domain of Kid-1. Transcriptional repressor activity of GAL4-Kid-1 fusion proteins maps to the KRAB-A domain. The KRAB-A domain of another zinc finger protein, ZNF2, also has repressor activity. Site-directed mutagenesis of conserved amino acids in this motif results in decreased repressor activity. Thus, we have established a functional significance for the KRAB-A domain, a consensus sequence common in zinc finger proteins.
MeSH Terms
Amino Acid Sequence
Animals
Cell Line
Chloramphenicol O-Acetyltransferase/biosynthesis,metabolism
Chlorocebus aethiops
Consensus Sequence
DNA-Binding Proteins/biosynthesis,genetics,metabolism
Fungal Proteins/biosynthesis,metabolism
Genetic Vectors
Kidney/metabolism
Kinetics
Kruppel-Like Transcription Factors
Molecular Sequence Data
Mutagenesis, Site-Directed
Polymerase Chain Reaction
Rats
Recombinant Fusion Proteins/biosynthesis,metabolism
Repressor Proteins/metabolism
Saccharomyces cerevisiae Proteins
Sequence Homology, Amino Acid
Transcription Factors/biosynthesis,genetics,metabolism
Transfection
Zinc Fingers/genetics
Chemicals
DNA-Binding Proteins
Fungal Proteins
GAL4 protein, S cerevisiae
Kruppel-Like Transcription Factors
Recombinant Fusion Proteins
Repressor Proteins
Saccharomyces cerevisiae Proteins
Transcription Factors
Zfp354a protein, rat
Chloramphenicol O-Acetyltransferase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Witzgall R
Medical Services, Massachusetts General Hospital, Charlestown 02129.
O'Leary E
Leaf A
Onaldi D
Bonventre J V
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