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PMID: 8978616 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Transcription activation at class II CAP-dependent promoters: two interactions between CAP and RNA polymerase.

Cell ·Vol. 87 ·No. 6 ·1996-12-13 ·Pages 1123-34

Niu W, Kim Y, Tau G, Heyduk T, Ebright RH

Abstract

At Class II catabolite activator protein (CAP)-dependent promoters, CAP activates transcription from a DNA site overlapping the DNA site for RNA polymerase. We show that transcription activation at Class II CAP-dependent promoters requires not only the previously characterized interaction between an activating region of CAP and the RNA polymerase alpha subunit C-terminal domain, but also an interaction between a second, promoter-class-specific activating region of CAP and the RNA polymerase alpha subunit N-terminal domain. We further show that the two interactions affect different steps in transcription initiation. Transcription activation at Class II CAP-dependent promoters provides a paradigm for understanding how an activator can make multiple interactions with the transcription machinery, each interaction being responsible for a specific mechanistic consequence.

MeSH Terms
Amino Acids/chemistry Binding Sites/genetics Cyclic AMP Receptor Protein/chemistry,genetics,metabolism DNA, Bacterial/genetics DNA-Directed RNA Polymerases/chemistry,metabolism Dimerization Electrochemistry Enzyme Activation/genetics Escherichia coli/enzymology,genetics Mutagenesis/physiology Promoter Regions, Genetic/genetics Protein Binding/physiology Protein Conformation Protein Structure, Tertiary Transcription, Genetic/physiology
Chemicals
Amino Acids Cyclic AMP Receptor Protein DNA, Bacterial DNA-Directed RNA Polymerases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Niu W
Department of Chemistry and Waksman Institute, Rutgers University, New Brunswick, New Jersey 08855, USA.
Kim Y
Tau G
Heyduk T
Ebright R H
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Article Info
Journal
Cell
Abbr.
Cell
ISSN
0092-8674
DOI
10.1016/s0092-8674(00)81806-1
Published
1996-12-13
Pages
1123-34
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC4430116
Subset
IM
Grants
NIGMS NIH HHS · R01 GM041376 · United States
NIGMS NIH HHS · R37 GM041376 · United States
NIGMS NIH HHS · GM50514 · United States
NIGMS NIH HHS · R01 GM050514 · United States
NIGMS NIH HHS · GM41376 · United States
External Links
PubMed source DOI: 10.1016/s0092-8674(00)81806-1 PMC Full Text View journal details
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