Home LiteratureArticle Details
PMID: 8975932 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Identification of a family of intimins common to Escherichia coli causing attaching-effacing lesions in rabbits, humans, and swine.

Infection and immunity ·Vol. 65 ·No. 1 ·1997-01-00 ·Pages 320-6

Agin TS, Wolf MK

Abstract

Intimin, an outer membrane protein encoded by eaeA that mediates close attachment of enteropathogenic bacteria to apical surfaces of epithelial cells, is required for formation of the attaching-effacing lesions and for full pathogenesis of the bacteria. Analysis of the eaeA sequence indicates that there is a high degree of homology at the N termini but less at the C termini of intimins. Antisera specific for the C-terminal third of RDEC-1 intimin, used to screen outer membrane proteins from 50 rabbit enteropathogenic Escherichia coli (EPEC), human EPEC, and human enterohemorrhagic E. coli (EHEC) strains, identified cross-reactive intimins from 24 isolates. Sequence analysis of the eaeA genes from human EPEC O111 and EHEC O26 isolates indicates that their intimins have C termini nearly identical to that of RDEC-1 intimin. Our results suggest that there are at least three families of related intimins and that the presence of intimin similar to that of RDEC-1 is not restricted by serogroup or host specificity.

MeSH Terms
Adhesins, Bacterial Animals Antibodies, Bacterial Bacterial Adhesion/genetics Bacterial Outer Membrane Proteins/genetics,immunology Carrier Proteins Cross Reactions Diarrhea/etiology Escherichia coli/classification,genetics,immunology,pathogenicity Escherichia coli Proteins Genes, Bacterial Humans Molecular Sequence Data Multigene Family Rabbits Serotyping Species Specificity Swine
Chemicals
Adhesins, Bacterial Antibodies, Bacterial Bacterial Outer Membrane Proteins Carrier Proteins Escherichia coli Proteins eaeA protein, E coli
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Agin T S
Department of Gastroenterology, Walter Reed Army Institute of Research, Washington, D.C. 20307-5100, USA.
Wolf M K
References (47)
47 references, click to expand
  1. A genetic locus of enterocyte effacement conserved among diverse enterobacterial pathogens.
    Proc Natl Acad Sci U S A. 1995 Feb 28;92(5):1664-8 PMID: 7878036
  2. Virulence properties and attaching-effacing activity of Escherichia coli O45 from swine postweaning diarrhea.
    Infect Immun. 1994 Oct;62(10):4153-9 PMID: 7927670
  3. Diarrhea due to Escherichia coli in the rabbit: a novel mechanism.
    J Infect Dis. 1977 Mar;135(3):454-62 PMID: 321703
  4. Scanning and transmission electron microscopic study of Escherichia coli O15 (RDEC-1) enteric infection in rabbits.
    Infect Immun. 1978 Feb;19(2):686-94 PMID: 344219
  5. Hemorrhagic colitis associated with a rare Escherichia coli serotype.
    N Engl J Med. 1983 Mar 24;308(12):681-5 PMID: 6338386
  6. Pathogenic properties of Escherichia coli strains isolated from diarrheic commercial rabbits.
    J Clin Microbiol. 1984 Jul;20(1):34-9 PMID: 6378965
  7. The association between idiopathic hemolytic uremic syndrome and infection by verotoxin-producing Escherichia coli.
    J Infect Dis. 1985 May;151(5):775-82 PMID: 3886804
  8. The diarrheal response of humans to some classic serotypes of enteropathogenic Escherichia coli is dependent on a plasmid encoding an enteroadhesiveness factor.
    J Infect Dis. 1985 Sep;152(3):550-9 PMID: 2863318
  9. Natural and experimental infection with an attaching and effacing strain of Escherichia coli in calves.
    Infect Immun. 1986 Aug;53(2):339-46 PMID: 3525410
  10. Natural infection with an attaching and effacing Escherichia coli in a diarrheic puppy.
    Can J Vet Res. 1988 Apr;52(2):280-2 PMID: 3285983
  11. Characterization of the plasmid from Escherichia coli RDEC-1 that mediates expression of adhesin AF/R1 and evidence that AF/R1 pili promote but are not essential for enteropathogenic disease.
    Infect Immun. 1988 Aug;56(8):1846-57 PMID: 2899552
  12. Efficacy of a cell extract from Actinobacillus (Haemophilus) pleuropneumoniae serotype 1 against disease in swine.
    Infect Immun. 1990 Feb;58(2):358-65 PMID: 2298483
  13. A plasmid-encoded regulatory region activates chromosomal eaeA expression in enteropathogenic Escherichia coli.
    Infect Immun. 1995 May;63(5):1767-76 PMID: 7729884
  14. Identification of EaeA protein in the outer membrane of attaching and effacing Escherichia coli O45 from pigs.
    FEMS Microbiol Lett. 1995 Jun 15;129(2-3):237-42 PMID: 7607406
  15. Protein secretion by enteropathogenic Escherichia coli is essential for transducing signals to epithelial cells.
    Proc Natl Acad Sci U S A. 1995 Aug 15;92(17):7991-5 PMID: 7644526
  16. Enteropathogenic Escherichia coli contains a putative type III secretion system necessary for the export of proteins involved in attaching and effacing lesion formation.
    Proc Natl Acad Sci U S A. 1995 Aug 15;92(17):7996-8000 PMID: 7644527
  17. Molecular characterization of a carboxy-terminal eukaryotic-cell-binding domain of intimin from enteropathogenic Escherichia coli.
    Infect Immun. 1995 Nov;63(11):4323-8 PMID: 7591066
  18. Truncated enterohemorrhagic Escherichia coli (EHEC) O157:H7 intimin (EaeA) fusion proteins promote adherence of EHEC strains to HEp-2 cells.
    Infect Immun. 1996 Jun;64(6):2225-33 PMID: 8675331
  19. A pathogenic bacterium triggers epithelial signals to form a functional bacterial receptor that mediates actin pseudopod formation.
    EMBO J. 1996 Jun 3;15(11):2613-24 PMID: 8654358
  20. EspA, a protein secreted by enteropathogenic Escherichia coli, is required to induce signals in epithelial cells.
    Mol Microbiol. 1996 Apr;20(2):313-23 PMID: 8733230
  21. Escherichia coli O157:H7 in microbial flora of sheep.
    J Clin Microbiol. 1996 Feb;34(2):431-3 PMID: 8789031
  22. Characterization of the eaeA gene from rabbit enteropathogenic Escherichia coli strain RDEC-1 and comparison to other eaeA genes from bacteria that cause attaching-effacing lesions.
    FEMS Microbiol Lett. 1996 Nov 1;144(2-3):249-58 PMID: 8900070
  23. A genetic locus of enteropathogenic Escherichia coli necessary for the production of attaching and effacing lesions on tissue culture cells.
    Proc Natl Acad Sci U S A. 1990 Oct;87(20):7839-43 PMID: 2172966
  24. Clonal relationships among classic enteropathogenic Escherichia coli (EPEC) belong to different O groups.
    J Infect Dis. 1990 Jul;162(1):76-81 PMID: 2192012
  25. The role of the eae gene of enterohemorrhagic Escherichia coli in intimate attachment in vitro and in a porcine model.
    J Clin Invest. 1993 Sep;92(3):1418-24 PMID: 8376595
  26. Experimental infection of newborn pigs with an attaching and effacing Escherichia coli O45:K"E65" strain.
    Infect Immun. 1991 Mar;59(3):814-21 PMID: 1997432
  27. Attaching and effacing Escherichia coli infections in calves, pigs, lambs, and dogs.
    J Vet Diagn Invest. 1989 Jan;1(1):6-11 PMID: 2488649
  28. Inhibition of localized adhesion of enteropathogenic Escherichia coli to HEp-2 cells by immunoglobulin and oligosaccharide fractions of human colostrum and breast milk.
    J Infect Dis. 1991 Jun;163(6):1247-55 PMID: 1903799
  29. Plasmid and chromosomal elements involved in the pathogenesis of attaching and effacing Escherichia coli.
    Infect Immun. 1991 Nov;59(11):3869-75 PMID: 1937746
  30. HeLa cell adherence, actin aggregation, and invasion by nonenteropathogenic Escherichia coli possessing the eae gene.
    Infect Immun. 1991 Nov;59(11):3924-9 PMID: 1682254
  31. The eae gene of enteropathogenic Escherichia coli encodes a 94-kilodalton membrane protein, the expression of which is influenced by the EAF plasmid.
    Infect Immun. 1991 Dec;59(12):4302-9 PMID: 1682258
  32. Cloning and characterization of the eae gene of enterohaemorrhagic Escherichia coli O157:H7.
    Mol Microbiol. 1992 Feb;6(3):411-7 PMID: 1552854
  33. Cloning and nucleotide sequence of the eae gene homologue from enterohemorrhagic Escherichia coli serotype O157:H7.
    FEMS Microbiol Lett. 1992 Feb 1;70(1):63-8 PMID: 1577255
  34. Enteropathogenic Escherichia coli.
    Infect Immun. 1992 Oct;60(10):3953-61 PMID: 1398907
  35. Sharing of virulence-associated properties at the phenotypic and genetic levels between enteropathogenic Escherichia coli and Hafnia alvei.
    J Med Microbiol. 1992 Nov;37(5):310-4 PMID: 1433251
  36. A plasmid-encoded type IV fimbrial gene of enteropathogenic Escherichia coli associated with localized adherence.
    Mol Microbiol. 1992 Nov;6(22):3427-37 PMID: 1362446
  37. Clonal relationships among Escherichia coli strains that cause hemorrhagic colitis and infantile diarrhea.
    Infect Immun. 1993 May;61(5):1619-29 PMID: 7682992
  38. Attaching and effacing locus of a Citrobacter freundii biotype that causes transmissible murine colonic hyperplasia.
    Infect Immun. 1993 Jun;61(6):2486-92 PMID: 8500884
  39. A second chromosomal gene necessary for intimate attachment of enteropathogenic Escherichia coli to epithelial cells.
    J Bacteriol. 1993 Aug;175(15):4670-80 PMID: 8393004
  40. Role of the eaeA gene in experimental enteropathogenic Escherichia coli infection.
    J Clin Invest. 1993 Sep;92(3):1412-7 PMID: 8376594
  41. The eae gene of Citrobacter freundii biotype 4280 is necessary for colonization in transmissible murine colonic hyperplasia.
    Infect Immun. 1993 Nov;61(11):4654-61 PMID: 8406863
  42. Role of Shiga-like toxin I in bacterial enteritis: comparison between isogenic Escherichia coli strains induced in rabbits.
    Gastroenterology. 1994 Feb;106(2):306-17 PMID: 8299898
  43. Characterization of the C-terminal domains of intimin-like proteins of enteropathogenic and enterohemorrhagic Escherichia coli, Citrobacter freundii, and Hafnia alvei.
    Infect Immun. 1994 May;62(5):1835-42 PMID: 8168946
  44. Sequence heterogeneity of the eae gene and detection of verotoxin-producing Escherichia coli using serotype-specific primers.
    Epidemiol Infect. 1994 Jun;112(3):449-61 PMID: 8005211
  45. Escherichia coli serogroup O111 includes several clones of diarrheagenic strains with different virulence properties.
    Infect Immun. 1994 Aug;62(8):3282-8 PMID: 8039899
  46. Adhesion and its role in the virulence of enteropathogenic Escherichia coli.
    Clin Microbiol Rev. 1994 Apr;7(2):152-73 PMID: 8055465
  47. Attachment and penetration of Escherichia coli into intestinal epithelium of the ileum in newborn pigs.
    Am J Pathol. 1969 Sep;56(3):371-92 PMID: 4898367
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.65.1.320-326.1997
Published
1997-01-00
Pages
320-6
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC174596
Subset
IM
Databases
GENBANK
L11691, L29509, M34051, U59502, U60002, U62655, U62656, U62657, Z11541
External Links
PubMed source DOI: 10.1128/iai.65.1.320-326.1997 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2