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PMID: 8406863 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The eae gene of Citrobacter freundii biotype 4280 is necessary for colonization in transmissible murine colonic hyperplasia.

Infection and immunity ·Vol. 61 ·No. 11 ·1993-11-00 ·Pages 4654-61

Schauer DB, Falkow S

Abstract

Transmissible murine colonic hyperplasia is characterized by proliferation of anchored stem cells in the mucosa of the descending colon of laboratory mice and is caused by Citrobacter freundii biotype 4280. This bacterium produces attaching and effacing lesions in the descending colon prior to the onset of gross hyperplasia. By mutational analysis, the chromosomal eae gene of C. freundii biotype 4280 was shown to be necessary for colonic colonization. Conversely, bacteria cured of a 65-kb plasmid, which was identified in C. freundii biotype 4280, were not attenuated for colonic colonization or for the induction of colonic hyperplasia.

MeSH Terms
Adhesins, Bacterial Animals Bacterial Outer Membrane Proteins/genetics Carrier Proteins Citrobacter freundii/genetics,pathogenicity Colon/microbiology,pathology Enterobacteriaceae Infections/microbiology,pathology Escherichia coli Proteins Gene Deletion Genes, Bacterial Hyperplasia Mice Mice, Inbred C3H Mutation Plasmids
Chemicals
Adhesins, Bacterial Bacterial Outer Membrane Proteins Carrier Proteins Escherichia coli Proteins eaeA protein, E coli
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Schauer D B
Department of Microbiology and Immunology, Stanford University School of Medicine, California 94305-5402.
Falkow S
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.61.11.4654-4661.1993
Published
1993-11-00
Pages
4654-61
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC281217
Subset
IM
Grants
NIAID NIH HHS · AI26195 · United States
External Links
PubMed source DOI: 10.1128/iai.61.11.4654-4661.1993 PMC Full Text View journal details
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