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PMID: 8953766 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

det1, cop1, and cop9 mutations cause inappropriate expression of several gene sets.

The Plant cell ·Vol. 8 ·No. 11 ·1996-11-00 ·Pages 1951-9

Mayer R, Raventos D, Chua NH

Abstract

Genetic studies using Arabidopsis offer a promising approach to investigate the mechanisms of light signal transduction during seedling development. Several mutants, called det/cop, have been isolated based on their deetiolated/constitutive photomorphogenic phenotypes in the dark. This study examines the specificity of the det/cop mutations with respect to their effects on genes regulated by other signal transduction pathways. Steady state mRNA levels of a number of differently regulated gene sets were compared between mutants and the wild type. We found that det2, cop2, cop3, and cop4 mutants displayed a gene expression pattern similar to that of the wild type. By contrast, det1, cop1, and cop9 mutations exhibited pleiotropic effects. In addition to light-responsive genes, genes normally inducible by plant pathogens, hypoxia, and developmental programs were inappropriately expressed in these mutants. Our data provide evidence that DET1, COP1, and COP9 most likely act as negative regulators of several sets of genes, not just those involved in light-regulated seedling development.

MeSH Terms
Arabidopsis Arabidopsis Proteins COP9 Signalosome Complex Carrier Proteins/genetics Gene Expression Regulation, Plant Intracellular Signaling Peptides and Proteins Multiprotein Complexes Mutagenesis, Site-Directed Nuclear Proteins/genetics Peptide Hydrolases Plant Growth Regulators Plant Proteins/genetics Proteins Seeds/genetics,growth & development Signal Transduction Ubiquitin-Protein Ligases
Chemicals
Arabidopsis Proteins Carrier Proteins DET1 protein, Arabidopsis Intracellular Signaling Peptides and Proteins Multiprotein Complexes Nuclear Proteins Plant Growth Regulators Plant Proteins Proteins AT2G32950 protein, Arabidopsis Ubiquitin-Protein Ligases Peptide Hydrolases COP9 Signalosome Complex
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mayer R
Laboratory of Plant Molecular Biology, Rockefeller University, New York, New York 10021-6399, USA.
Raventos D
Chua N H
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.8.11.1951
Published
1996-11-00
Pages
1951-9
Language
English
Region
England
NLM ID
9208688
PMCID
PMC161326
Subset
IM
Grants
NIGMS NIH HHS · GM44640 · United States
External Links
PubMed source DOI: 10.1105/tpc.8.11.1951 PMC Full Text View journal details
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