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PMID: 8929426 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Translational machinery in dendrites of hippocampal neurons in culture.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 16 ·No. 22 ·1996-11-15 ·Pages 7171-81

Tiedge H, Brosius J

Abstract

In neurons, several mRNAs are selectively delivered to dendritic domains where they are presumably translated by local protein synthetic machinery. Although electron microscopy has identified polyribosomes in dendrites, in particular in postsynaptic dendritic compartments, the functional composition of the local protein synthetic apparatus and the scope of its translational capacity have not been analyzed. To ascertain the translational competence of dendrites, we have probed hippocampal neurons in primary culture for various integral and associated factors of the translational apparatus. We report here that dendrites of such neurons are equipped with a spectrum of translational machinery components, including ribosomes, tRNAs, initiation and elongation factors, and elements of the cotranslational signal recognition mechanism. These components are differentially and nonuniformly distributed in dendritic arbors. Their dendritic location illustrates the soma-independent potential of dendrites to synthesize selected proteins in local domains.

MeSH Terms
Animals Cells, Cultured/chemistry,physiology,ultrastructure Dendrites/chemistry,physiology Fluorescent Antibody Technique Hippocampus/cytology In Situ Hybridization Neurons/cytology,physiology,ultrastructure Protein Biosynthesis/physiology Protein Sorting Signals/analysis RNA, Messenger/analysis RNA, Transfer/analysis Rats Ribosomes/physiology
Chemicals
Protein Sorting Signals RNA, Messenger RNA, Transfer
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tiedge H
Department of Pharmacology, State University of New York Health Science Center at Brooklyn 11203, USA.
Brosius J
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
0270-6474
Published
1996-11-15
Pages
7171-81
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6578937
Subset
IM
Grants
NIMH NIH HHS · MH38819 · United States
NINDS NIH HHS · NS23094 · United States
NINDS NIH HHS · NS34158 · United States
External Links
PubMed source PMC Full Text View journal details
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