Home LiteratureArticle Details
PMID: 8917598 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

A cdc5+ homolog of a higher plant, Arabidopsis thaliana.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 93 ·No. 23 ·1996-11-12 ·Pages 13371-6

Hirayama T, Shinozaki K

Abstract

We cloned and characterized a cDNA corresponding to a cdc5+ homolog of the higher plant, Arabidopsis thaliana. The cDNA, named AtCDC5 cDNA, encodes a polypeptide of 844 amino acid residues. The amino acid sequence of N-terminal one-fourth region of the predicted protein bears significant similarity to that of Schizosaccharomyces pombe Cdc5 and Myb-related proteins. Overexpression of the AtCDC5 cDNA in S. pombe cells is able to complement the growth defective phenotype of a cdc5 temperature-sensitive mutant. These results indicate that the AtCDC5 gene is a plant counterpart of S. pombe cdc5+. This is the first report of a cdc5(+)-like gene in a multicellular organism. We also demonstrated that a recombinant AtCDC5 protein possesses a sequence specific DNA binding activity (CTCAGCG) and the AtCDC5 gene is expressed extensively in shoot and root meristems. In addition, we cloned a PCR fragment corresponding to the DNA binding domain of human Cdc5-like protein. These results strongly suggest that Cdc5-like protein exists in all eukaryotes and may function in cell cycle regulation.

MeSH Terms
Amino Acid Sequence Animals Arabidopsis/genetics,metabolism Arabidopsis Proteins Base Sequence Binding Sites Cell Cycle Proteins/biosynthesis,chemistry,metabolism DNA Primers Drosophila Genetic Complementation Test Glutathione Transferase/biosynthesis Humans In Situ Hybridization Molecular Sequence Data Oligodeoxyribonucleotides Plant Proteins/biosynthesis,chemistry Polymerase Chain Reaction Proto-Oncogene Proteins/chemistry Proto-Oncogene Proteins c-myb Recombinant Fusion Proteins/biosynthesis Schizosaccharomyces/genetics,growth & development Schizosaccharomyces pombe Proteins Sequence Homology, Amino Acid Trans-Activators/chemistry Transcription Factors/biosynthesis,chemistry,metabolism
Chemicals
ATCDC5 protein, Arabidopsis Arabidopsis Proteins Cell Cycle Proteins DNA Primers Oligodeoxyribonucleotides Plant Proteins Proto-Oncogene Proteins Proto-Oncogene Proteins c-myb Recombinant Fusion Proteins Schizosaccharomyces pombe Proteins Trans-Activators Transcription Factors cdc5 protein, S pombe Glutathione Transferase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hirayama T
Laboratory of Plant Molecular Biology, Tsukuba Life Science Center, Institute of Physical and Chemical Research (RIKEN), Ibaraki, Japan.
Shinozaki K
References (51)
51 references, click to expand
  1. Isolation of the proto-oncogene c-myb from D. melanogaster.
    Cell. 1985 Jun;41(2):449-56 PMID: 3921261
  2. Cell cycle regulation in the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe.
    Annu Rev Cell Biol. 1991;7:227-56 PMID: 1809348
  3. Human c-myb protooncogene: nucleotide sequence of cDNA and organization of the genomic locus.
    Proc Natl Acad Sci U S A. 1986 Dec;83(24):9636-40 PMID: 3540945
  4. Cell cycle regulation of SW15 is required for mother-cell-specific HO transcription in yeast.
    Cell. 1987 May 22;49(4):549-58 PMID: 3552251
  5. The regulatory c1 locus of Zea mays encodes a protein with homology to myb proto-oncogene products and with structural similarities to transcriptional activators.
    EMBO J. 1987 Dec 1;6(12):3553-8 PMID: 3428265
  6. Identification of a DNA binding factor involved in cell-cycle control of the yeast HO gene.
    Cell. 1989 Apr 7;57(1):21-9 PMID: 2649246
  7. Checkpoints: controls that ensure the order of cell cycle events.
    Science. 1989 Nov 3;246(4930):629-34 PMID: 2683079
  8. Universal control mechanism regulating onset of M-phase.
    Nature. 1990 Apr 5;344(6266):503-8 PMID: 2138713
  9. Regulation of mitosis by cyclic accumulation of p80cdc25 mitotic inducer in fission yeast.
    Nature. 1990 Apr 5;344(6266):549-52 PMID: 2320127
  10. Substrates for p34cdc2: in vivo veritas?
    Cell. 1990 May 18;61(4):549-51 PMID: 2188729
  11. nmt1 of fission yeast. A highly transcribed gene completely repressed by thiamine.
    J Biol Chem. 1990 Jul 5;265(19):10857-64 PMID: 2358444
  12. Regulation of cell cycle-dependent gene expression in yeast.
    J Biol Chem. 1990 Aug 25;265(24):14057-60 PMID: 2201678
  13. Cell cycle control of DNA synthesis in budding yeast.
    Nucleic Acids Res. 1992 May 25;20(10):2403-10 PMID: 1598198
  14. SWI6 protein is required for transcription of the periodically expressed DNA synthesis genes in budding yeast.
    Nature. 1992 Jun 11;357(6378):505-8 PMID: 1608450
  15. A central role for SWI6 in modulating cell cycle Start-specific transcription in yeast.
    Nature. 1992 Jun 11;357(6378):508-13 PMID: 1608451
  16. Spatial pattern of cdc2 expression in relation to meristem activity and cell proliferation during plant development.
    Proc Natl Acad Sci U S A. 1992 Aug 15;89(16):7360-4 PMID: 1502145
  17. The Arabidopsis functional homolog of the p34cdc2 protein kinase.
    Plant Cell. 1991 May;3(5):531-40 PMID: 1840925
  18. Novel protein kinase of Arabidopsis thaliana (APK1) that phosphorylates tyrosine, serine and threonine.
    Plant Mol Biol. 1992 Nov;20(4):653-62 PMID: 1450380
  19. A new cdc gene required for S phase entry of Schizosaccharomyces pombe encodes a protein similar to the cdc 10+ and SWI4 gene products.
    EMBO J. 1992 Dec;11(13):4923-32 PMID: 1464317
  20. Two functional soybean genes encoding p34cdc2 protein kinases are regulated by different plant developmental pathways.
    Proc Natl Acad Sci U S A. 1993 Feb 1;90(3):943-7 PMID: 8430109
  21. cdc2a expression in Arabidopsis is linked with competence for cell division.
    Plant Cell. 1993 Dec;5(12):1711-23 PMID: 8305869
  22. An Arabidopsis myb homolog is induced by dehydration stress and its gene product binds to the conserved MYB recognition sequence.
    Plant Cell. 1993 Nov;5(11):1529-39 PMID: 8312738
  23. The Schizosaccharomyces pombe cdc5+ gene encodes an essential protein with homology to c-Myb.
    EMBO J. 1994 Jan 15;13(2):471-83 PMID: 8313892
  24. Patterns of cell division revealed by transcriptional regulation of genes during the cell cycle in plants.
    EMBO J. 1994 Feb 1;13(3):616-24 PMID: 8313906
  25. Cloning of a family of cyclins from Arabidopsis thaliana.
    Biochim Biophys Acta. 1994 May 17;1218(1):115-8 PMID: 8193159
  26. Cloning of four cyclins from maize indicates that higher plants have three structurally distinct groups of mitotic cyclins.
    Proc Natl Acad Sci U S A. 1994 Jul 19;91(15):7375-9 PMID: 8041798
  27. Ordering S phase and M phase in the cell cycle.
    Cell. 1994 Nov 18;79(4):547-50 PMID: 7954820
  28. G1 phase progression: cycling on cue.
    Cell. 1994 Nov 18;79(4):551-5 PMID: 7954821
  29. Solution structure of a specific DNA complex of the Myb DNA-binding domain with cooperative recognition helices.
    Cell. 1994 Nov 18;79(4):639-48 PMID: 7954830
  30. Developmental expression of the arabidopsis cyclin gene cyc1At.
    Plant Cell. 1994 Dec;6(12):1763-74 PMID: 7866022
  31. A family of cyclin D homologs from plants differentially controlled by growth regulators and containing the conserved retinoblastoma protein interaction motif.
    Plant Cell. 1995 Jan;7(1):85-103 PMID: 7696881
  32. A gene encoding a phosphatidylinositol-specific phospholipase C is induced by dehydration and salt stress in Arabidopsis thaliana.
    Proc Natl Acad Sci U S A. 1995 Apr 25;92(9):3903-7 PMID: 7732004
  33. Transcriptional regulation during the mammalian cell cycle.
    Trends Genet. 1995 May;11(5):173-8 PMID: 7785076
  34. cycMs3, a novel B-type alfalfa cyclin gene, is induced in the G0-to-G1 transition of the cell cycle.
    Plant Cell. 1995 Jun;7(6):759-71 PMID: 7647566
  35. The D-type alfalfa cyclin gene cycMs4 complements G1 cyclin-deficient yeast and is induced in the G1 phase of the cell cycle.
    Plant Cell. 1995 Nov;7(11):1847-57 PMID: 8535138
  36. Complementation of a yeast cell cycle mutant by an alfalfa cDNA encoding a protein kinase homologous to p34cdc2.
    Proc Natl Acad Sci U S A. 1991 Mar 1;88(5):1636-40 PMID: 2000373
  37. Coordination of expression of DNA synthesis genes in budding yeast by a cell-cycle regulated trans factor.
    Nature. 1991 Mar 21;350(6315):247-50 PMID: 2005980
  38. The role of CDC28 and cyclins during mitosis in the budding yeast S. cerevisiae.
    Cell. 1991 Apr 5;65(1):145-61 PMID: 1849457
  39. A cyclin B homolog in S. cerevisiae: chronic activation of the Cdc28 protein kinase by cyclin prevents exit from mitosis.
    Cell. 1991 Apr 5;65(1):163-74 PMID: 1849458
  40. Isolation and characterization of cDNA clones encoding a functional p34cdc2 homologue from Zea mays.
    Proc Natl Acad Sci U S A. 1991 Apr 15;88(8):3377-81 PMID: 2014258
  41. Isolation and characterization of cDNA clones for plant cyclins.
    EMBO J. 1991 Sep;10(9):2681-8 PMID: 1831125
  42. Definition of a consensus sequence for peptide substrate recognition by p44mpk, the meiosis-activated myelin basic protein kinase.
    J Biol Chem. 1991 Aug 15;266(23):15180-4 PMID: 1907971
  43. A myb gene required for leaf trichome differentiation in Arabidopsis is expressed in stipules.
    Cell. 1991 Nov 1;67(3):483-93 PMID: 1934056
  44. Changes in a SWI4,6-DNA-binding complex occur at the time of HO gene activation in yeast.
    Genes Dev. 1991 Nov;5(11):2000-13 PMID: 1936990
  45. Identification of two cell-cycle-controlling cdc2 gene homologs in Arabidopsis thaliana.
    Gene. 1991 Sep 15;105(2):159-65 PMID: 1937013
  46. Control of root growth and development by cyclin expression.
    Nature. 1996 Apr 11;380(6574):520-3 PMID: 8606769
  47. Alfalfa cyclins: differential expression during the cell cycle and in plant organs.
    Plant Cell. 1992 Dec;4(12):1531-8 PMID: 1307238
  48. Identification of substrate recognition determinants for human ERK1 and ERK2 protein kinases.
    J Biol Chem. 1991 Nov 25;266(33):22159-63 PMID: 1939237
  49. Control of DNA synthesis genes in fission yeast by the cell-cycle gene cdc10+.
    Nature. 1992 Jan 30;355(6359):449-53 PMID: 1734281
  50. Genes regulating the plant cell cycle: isolation of a mitotic-like cyclin from Arabidopsis thaliana.
    Proc Natl Acad Sci U S A. 1992 Apr 15;89(8):3295-9 PMID: 1373494
  51. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.93.23.13371
Published
1996-11-12
Pages
13371-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC24100
Subset
IM
Databases
GENBANK
D58424, D85423
External Links
PubMed source DOI: 10.1073/pnas.93.23.13371 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2