A purified mariner transposase is sufficient to mediate transposition in vitro.

The EMBO journal ·Vol. 15 ·No. 19 ·1996-10-01 ·Pages 5470-9

Lampe DJ, Churchill ME, Robertson HM

Abstract

Mariners are a widespread and diverse family of animal transposons. Extremely similar mariners of the irritans subfamily are present in the genomes of three divergent insect host species, which strongly suggests that species-specific host factors are unnecessary for mobility. We tested this hypothesis by examining the activity of a purified transposase from one of these elements (Himar1) present in the horn fly, Haematobia irritans. Himar1 transposase was sufficient to reproduce transposition faithfully in an in vitro inter-plasmid transposition reaction. Further analyses showed that Himar1 transposase binds to the inverted terminal repeat sequences of its cognate transposon and mediates 5' and 3' cleavage of the element termini. Independence of species-specific host factors helps to explain why mariners have such a broad distribution and why they are capable of horizontal transfer between species.

MeSH Terms

Animals Base Sequence Chlorides/pharmacology DNA/metabolism DNA Footprinting DNA Nucleotidyltransferases/chemistry,isolation & purification,metabolism,pharmacology DNA Transposable Elements/physiology Manganese Compounds/pharmacology Molecular Sequence Data Molecular Weight Muscidae/enzymology,genetics Phylogeny Recombinant Fusion Proteins/isolation & purification,metabolism Transposases

Chemicals

Chlorides DNA Transposable Elements Manganese Compounds Recombinant Fusion Proteins DNA DNA Nucleotidyltransferases Transposases manganese chloride

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Lampe D J

Department of Entomology, University of Illinois at Urbana-Champaign, 61801, USA.

Churchill M E

Robertson H M

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35 references, click to expand

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Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1996-10-01

Pages

5470-9

Language

English

Region

England

NLM ID

8208664

PMCID

PMC452289

Subset

Grants

NCRR NIH HHS · P41 RR012408 · United States

NCRR NIH HHS · P41 RR012408-080045 · United States

Databases

GENBANK

U11641, U11652

Corrections

ErratumIn

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PubMed source PMC Full Text View journal details

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A purified mariner transposase is sufficient to mediate transposition in vitro.

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