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PMID: 8887550 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Rho 1 GTPase activates the (1-3)beta-D-glucan synthase and is involved in Schizosaccharomyces pombe morphogenesis.

The EMBO journal ·Vol. 15 ·No. 17 ·1996-09-02 ·Pages 4584-91

Arellano M, Durán A, Pérez P

Abstract

The Schizosaccharomyces pombe Cdc42 and Rho1 GTPases were tested for their ability to complement the cwg2-1 mutant phenotype of a decrease in (1-3)beta-D-glucan synthase activity when grown at the non-permissive temperature. Only Rho1 is able to partly complement the defect in glucan synthase associated with the cwg2-1 mutation. Moreover, overexpression of the rho1 gene in wild-type S.pombe cells causes aberrant morphology with loss of polarity and cells with several septa. Under this condition (1-3)beta-D-glucan synthase activity is increased four times, but is still dependent on GTP. When S.pombe is transformed with constitutively active rho1 mutant alleles (rho1-G15V or rho1-Q64L), cells stop growing and show a very thick cell wall with hardly any septum. Under this condition the level of (1-3)beta-D-glucan synthase activity is at least 20 times higher than wild-type and is independent of GTP. Neither cdc42+ nor the cdc42-V12G or cdc42-Q61L constitutively active mutant alleles affect (1-3)beta-D-glucan synthase activity when overexpressed in S.pombe. Cells overproducing Rho1 are hypersensitive to inhibitors of cell wall biosynthesis or to cell wall degrading enzymes. We conclude that Rho1 GTPase directly activates (1-3)beta-D-glucan synthase and regulates S.pombe morphogenesis.

MeSH Terms
Cell Cycle Proteins/genetics Enzyme Activation Fungal Proteins/metabolism GTP Phosphohydrolases/metabolism GTP-Binding Proteins/genetics,metabolism Genetic Complementation Test Glucosyltransferases/metabolism Membrane Proteins Microscopy, Electron Phenotype Schizosaccharomyces/enzymology,growth & development,ultrastructure Schizosaccharomyces pombe Proteins Transformation, Genetic cdc42 GTP-Binding Protein, Saccharomyces cerevisiae rho GTP-Binding Proteins
Chemicals
Cell Cycle Proteins Fungal Proteins Membrane Proteins Schizosaccharomyces pombe Proteins Glucosyltransferases 1,3-beta-glucan synthase GTP Phosphohydrolases GTP-Binding Proteins cdc42 GTP-Binding Protein, Saccharomyces cerevisiae rho GTP-Binding Proteins rho1 protein, S pombe
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Arellano M
Instituto de Microbiología Bioquímica, Consejo Superior de Investigaciones Científicas and Universidad de Salamanca, Edificio Departamental, Spain.
Durán A
Pérez P
References (44)
44 references, click to expand
  1. Mitochondrial growth and DNA synthesis occur in the absence of nuclear DNA replication in fission yeast.
    J Cell Sci. 1990 Nov;97 ( Pt 3):509-16 PMID: 2074269
  2. Isolation and sequencing of two cDNA clones encoding Rho proteins from the fission yeast Schizosaccharomyces pombe.
    Gene. 1995 Mar 21;155(1):119-22 PMID: 7698654
  3. Isolation and characterization of Schizosaccharomyces pombe mutants defective in cell wall (1-3)beta-D-glucan.
    J Bacteriol. 1991 Jun;173(11):3456-62 PMID: 1828464
  4. Mutational analysis of CDC42Sc, a Saccharomyces cerevisiae gene that encodes a putative GTP-binding protein involved in the control of cell polarity.
    Mol Cell Biol. 1991 Jul;11(7):3537-44 PMID: 1904541
  5. Mutants in the S. cerevisiae PKC1 gene display a cell cycle-specific osmotic stability defect.
    J Cell Biol. 1992 Mar;116(5):1221-9 PMID: 1740473
  6. A homologue of the ras-related CDC42 gene from Schizosaccharomyces pombe.
    Gene. 1992 May 1;114(1):153-4 PMID: 1587480
  7. The osmotic integrity of the yeast cell requires a functional PKC1 gene product.
    Mol Cell Biol. 1992 Nov;12(11):4896-905 PMID: 1406668
  8. GTPase cascades choreographing cellular behavior: movement, morphogenesis, and more.
    Cell. 1995 Apr 7;81(1):1-4 PMID: 7720065
  9. Rho, rac, and cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia.
    Cell. 1995 Apr 7;81(1):53-62 PMID: 7536630
  10. Rho-related proteins: actin cytoskeleton and cell cycle.
    Curr Opin Genet Dev. 1995 Feb;5(1):24-30 PMID: 7749321
  11. Cloning and characterization of GNS1: a Saccharomyces cerevisiae gene involved in synthesis of 1,3-beta-glucan in vitro.
    J Bacteriol. 1995 Jun;177(11):3227-34 PMID: 7768822
  12. Shk1, a homolog of the Saccharomyces cerevisiae Ste20 and mammalian p65PAK protein kinases, is a component of a Ras/Cdc42 signaling module in the fission yeast Schizosaccharomyces pombe.
    Proc Natl Acad Sci U S A. 1995 Jun 20;92(13):6180-4 PMID: 7597098
  13. Characterization and gene cloning of 1,3-beta-D-glucan synthase from Saccharomyces cerevisiae.
    Eur J Biochem. 1995 Aug 1;231(3):845-54 PMID: 7649185
  14. Ste20-like protein kinases are required for normal localization of cell growth and for cytokinesis in budding yeast.
    Genes Dev. 1995 Aug 1;9(15):1817-30 PMID: 7649470
  15. Role for the Rho-family GTPase Cdc42 in yeast mating-pheromone signal pathway.
    Nature. 1995 Aug 24;376(6542):702-5 PMID: 7651520
  16. An essential role for Rho, Rac, and Cdc42 GTPases in cell cycle progression through G1.
    Science. 1995 Sep 1;269(5228):1270-2 PMID: 7652575
  17. Differential expression and function of two homologous subunits of yeast 1,3-beta-D-glucan synthase.
    Mol Cell Biol. 1995 Oct;15(10):5671-81 PMID: 7565718
  18. Fission yeast pak1+ encodes a protein kinase that interacts with Cdc42p and is involved in the control of cell polarity and mating.
    EMBO J. 1995 Dec 1;14(23):5908-19 PMID: 8846783
  19. A downstream target of RHO1 small GTP-binding protein is PKC1, a homolog of protein kinase C, which leads to activation of the MAP kinase cascade in Saccharomyces cerevisiae.
    EMBO J. 1995 Dec 1;14(23):5931-8 PMID: 8846785
  20. HKR1 encodes a cell surface protein that regulates both cell wall beta-glucan synthesis and budding pattern in the yeast Saccharomyces cerevisiae.
    J Bacteriol. 1996 Jan;178(2):477-83 PMID: 8550469
  21. Rho1p, a yeast protein at the interface between cell polarization and morphogenesis.
    Science. 1996 Apr 12;272(5259):277-9 PMID: 8602514
  22. Genetic analysis of the Saccharomyces cerevisiae RHO3 gene, encoding a rho-type small GTPase, provides evidence for a role in bud formation.
    Genetics. 1996 Feb;142(2):359-69 PMID: 8852836
  23. Identification of yeast Rho1p GTPase as a regulatory subunit of 1,3-beta-glucan synthase.
    Science. 1996 Apr 12;272(5259):279-81 PMID: 8602515
  24. Papulacandin B: an inhibitor of glucan synthesis in yeast spheroplasts.
    Eur J Biochem. 1979 Jul;97(2):345-51 PMID: 380990
  25. The S. cerevisiae structural gene for chitin synthase is not required for chitin synthesis in vivo.
    Cell. 1986 Jul 18;46(2):213-25 PMID: 2941152
  26. Regulation of fungal cell wall growth: a guanine nucleotide-binding, proteinaceous component required for activity of (1----3)-beta-D-glucan synthase.
    Proc Natl Acad Sci U S A. 1986 Aug;83(16):5808-12 PMID: 2942941
  27. Characterization of two members of the rho gene family from the yeast Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1987 Feb;84(3):779-83 PMID: 3543936
  28. The GTPase superfamily: conserved structure and molecular mechanism.
    Nature. 1991 Jan 10;349(6305):117-27 PMID: 1898771
  29. Yeast RHO3 and RHO4 ras superfamily genes are necessary for bud growth, and their defect is suppressed by a high dose of bud formation genes CDC42 and BEM1.
    Mol Cell Biol. 1992 Dec;12(12):5690-9 PMID: 1448099
  30. Genetic evidence for in vivo cross-specificity of the CaaX-box protein prenyltransferases farnesyltransferase and geranylgeranyltransferase-I in Saccharomyces cerevisiae.
    Mol Cell Biol. 1993 Jul;13(7):4260-75 PMID: 8321228
  31. Comparison of Schizosaccharomyces pombe expression systems.
    Nucleic Acids Res. 1993 Jun 25;21(12):2955-6 PMID: 8332516
  32. The Schizosaccharomyces pombe cwg2+ gene codes for the beta subunit of a geranylgeranyltransferase type I required for beta-glucan synthesis.
    EMBO J. 1993 Dec 15;12(13):5245-54 PMID: 8262067
  33. The SLT2 (MPK1) MAP kinase homolog is involved in polarized cell growth in Saccharomyces cerevisiae.
    J Cell Biol. 1993 Dec;123(6 Pt 2):1821-33 PMID: 8276900
  34. Cdc42p GTPase is involved in controlling polarized cell growth in Schizosaccharomyces pombe.
    Mol Cell Biol. 1994 Feb;14(2):1075-83 PMID: 8289788
  35. Suppression of yeast geranylgeranyl transferase I defect by alternative prenylation of two target GTPases, Rho1p and Cdc42p.
    Mol Biol Cell. 1993 Oct;4(10):1017-25 PMID: 8298188
  36. Subcellular localization of Cdc42p, a Saccharomyces cerevisiae GTP-binding protein involved in the control of cell polarity.
    Mol Biol Cell. 1993 Dec;4(12):1307-16 PMID: 8167411
  37. Protein prenylation in eukaryotic microorganisms: genetics, biology and biochemistry.
    Mol Microbiol. 1994 Jan;11(2):219-25 PMID: 8170384
  38. Growth site localization of Rho1 small GTP-binding protein and its involvement in bud formation in Saccharomyces cerevisiae.
    J Cell Biol. 1994 Jun;125(5):1077-93 PMID: 8195291
  39. Cooperative interaction of S. pombe proteins required for mating and morphogenesis.
    Cell. 1994 Oct 7;79(1):131-41 PMID: 7923372
  40. Characterization of the yeast (1-->6)-beta-glucan biosynthetic components, Kre6p and Skn1p, and genetic interactions between the PKC1 pathway and extracellular matrix assembly.
    J Cell Biol. 1994 Oct;127(2):567-79 PMID: 7929594
  41. A GTP-binding protein regulates the activity of (1-->3)-beta-glucan synthase, an enzyme directly involved in yeast cell wall morphogenesis.
    J Biol Chem. 1994 Dec 9;269(49):31267-74 PMID: 7983071
  42. Interactions among proteins involved in bud-site selection and bud-site assembly in Saccharomyces cerevisiae.
    J Biol Chem. 1995 Jan 13;270(2):626-30 PMID: 7822288
  43. Small GTP-binding proteins and the regulation of the actin cytoskeleton.
    Annu Rev Cell Biol. 1994;10:31-54 PMID: 7888179
  44. Molecular genetic analysis of fission yeast Schizosaccharomyces pombe.
    Methods Enzymol. 1991;194:795-823 PMID: 2005825
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1996-09-02
Pages
4584-91
Language
English
Region
England
NLM ID
8208664
PMCID
PMC452188
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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