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PMID: 7923372 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cooperative interaction of S. pombe proteins required for mating and morphogenesis.

Cell ·Vol. 79 ·No. 1 ·1994-10-07 ·Pages 131-41

Chang EC, Barr M, Wang Y, Jung V, Xu HP, Wigler MH

Abstract

We isolated two S. pombe genes, scd1 and scd2, that are required for normal morphology and mating. scd1 and scd2 are homologous to CDC24 and BEM1, respectively, of S. cerevisiae. Epistasis analyses indicate that scd2 and ras1 converge upon scd1, which, in turn, interacts with cdc42sp, a RHO-like GTPase. Studies with the yeast two-hybrid system indicate that scd2 forms complexes with both scd1 and cdc42sp. Furthermore, biochemical studies indicate that the interaction between scd1 and scd2 is direct. The yeast two-hybrid data further suggest that scd1, scd2, cdc42sp, and ras1, in its GTP-bound state, act cooperatively to form a protein complex.

Related Genes

cdc42sp ras1 scd1 scd2

MeSH Terms

Adaptor Proteins, Signal Transducing Amino Acid Sequence Base Sequence Cell Cycle Proteins/genetics,metabolism Cloning, Molecular Epistasis, Genetic Fungal Proteins/genetics,metabolism GTP-Binding Proteins/genetics,metabolism Genes, Fungal/genetics Genetic Complementation Test Guanine Nucleotide Exchange Factors Models, Genetic Molecular Sequence Data Morphogenesis Proto-Oncogene Proteins/genetics,metabolism Saccharomyces cerevisiae Proteins Schizosaccharomyces/genetics,metabolism,physiology Schizosaccharomyces pombe Proteins Sequence Analysis, DNA Sequence Deletion/physiology Sequence Homology, Amino Acid cdc42 GTP-Binding Protein, Saccharomyces cerevisiae ras Proteins

Chemicals

Adaptor Proteins, Signal Transducing CDC24 protein, S cerevisiae Cell Cycle Proteins Fungal Proteins Guanine Nucleotide Exchange Factors Proto-Oncogene Proteins Saccharomyces cerevisiae Proteins Schizosaccharomyces pombe Proteins scd1 protein, S pombe scd2 protein, S pombe BEM1 protein, S cerevisiae GTP-Binding Proteins RAS1 protein, S cerevisiae Ras1 protein, S pombe cdc42 GTP-Binding Protein, Saccharomyces cerevisiae ras Proteins

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Chang E C

Cold Spring Harbor Laboratory, New York 11724.

Barr M

Wang Y

Jung V

Xu H P

Wigler M H

Article Info

Journal

Cell

Abbr.

Cell

ISSN

0092-8674

DOI

10.1016/0092-8674(94)90406-5

Published

1994-10-07

Pages

131-41

Language

English

Region

United States

NLM ID

0413066

Subset

Databases

GENBANK

U12538, U12539

External Links

PubMed source DOI: 10.1016/0092-8674(94)90406-5 View journal details

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Cooperative interaction of S. pombe proteins required for mating and morphogenesis.

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