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PMID: 8855220 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Hepatitis B virus transactivator protein, HBx, associates with the components of TFIIH and stimulates the DNA helicase activity of TFIIH.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 93 ·No. 20 ·1996-10-01 ·Pages 10578-83

Qadri I, Conaway JW, Conaway RC, Schaack J, Siddiqui A

Abstract

Human hepatitis B virus genome encodes a protein, termed HBx, that is widely recognized as a transcriptional transactivator. While HBx does not directly bind cis-acting transcriptional control elements, it has been shown to associate with cellular proteins that bind DNA. Because HBx transactivated a large number of viral/cellular transcriptional control elements, we looked for its targets within the components of the basal transcriptional machinery. This search led to the identification of its interactions with TFIIH. Here, we show that HBx interacts with yeast and mammalian TFIIH complexes both in vitro and in vivo. These interactions between HBx and the components of TFIIH are supported by several lines of evidence including results from immunoprocedures and direct methods of measuring interactions. We have identified ERCC3 and ERCC2 DNA helicase subunits of holoenzyme TFIIH as targets of HBx interactions. Furthermore, the DNA helicase activity of purified TFIIH from rat liver and, individually, the ERCC2 component of TFIIH is stimulated in the presence of HBx. These observations suggest a role for HBx in transcription and DNA repair.

MeSH Terms
Animals Binding Sites Cell Line DNA Helicases/metabolism DNA Repair Enzyme Activation HeLa Cells Hepatitis B virus/genetics Humans Protein Binding Rats Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins TATA-Binding Protein Associated Factors Trans-Activators/genetics Transcription Factor TFIID Transcription Factor TFIIH Transcription Factors/metabolism Transcription Factors, TFII Transcriptional Activation Viral Regulatory and Accessory Proteins
Chemicals
Saccharomyces cerevisiae Proteins TAF6 protein, S cerevisiae TATA-Binding Protein Associated Factors Trans-Activators Transcription Factor TFIID Transcription Factors Transcription Factors, TFII Viral Regulatory and Accessory Proteins hepatitis B virus X protein Transcription Factor TFIIH DNA Helicases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Qadri I
Department of Microbiology, University of Colorado Health Sciences Center, Denver 80262, USA.
Conaway J W
Conaway R C
Schaack J
Siddiqui A
References (34)
34 references, click to expand
  1. Transcription factor IIE binds preferentially to RNA polymerase IIa and recruits TFIIH: a model for promoter clearance.
    Genes Dev. 1994 Mar 1;8(5):515-24 PMID: 7926747
  2. Review: hepatitis B virus X-gene product: a promiscuous transcriptional activator.
    J Med Virol. 1992 Feb;36(2):101-17 PMID: 1583465
  3. Hepatitis B virus HBx protein activates Ras-GTP complex formation and establishes a Ras, Raf, MAP kinase signaling cascade.
    Proc Natl Acad Sci U S A. 1994 Oct 25;91(22):10350-4 PMID: 7937954
  4. RNA polymerase transcription factor IIH holoenzyme from yeast.
    J Biol Chem. 1994 Nov 11;269(45):28044-8 PMID: 7961739
  5. Hepatitis B virus X protein interacts with a probable cellular DNA repair protein.
    J Virol. 1995 Feb;69(2):1107-14 PMID: 7815490
  6. Human RPB5, a subunit shared by eukaryotic nuclear RNA polymerases, binds human hepatitis B virus X protein and may play a role in X transactivation.
    EMBO J. 1995 Jan 3;14(1):143-50 PMID: 7828586
  7. The multifunctional TFIIH complex and transcriptional control.
    Trends Biochem Sci. 1994 Nov;19(11):504-8 PMID: 7855896
  8. Hepatitis B virus transactivator protein X interacts with the TATA-binding protein.
    Proc Natl Acad Sci U S A. 1995 Feb 14;92(4):1003-7 PMID: 7862623
  9. The requirement for the basal transcription factor IIE is determined by the helical stability of promoter DNA.
    EMBO J. 1995 Feb 15;14(4):810-9 PMID: 7882984
  10. The RNA polymerase II holoenzyme and its implications for gene regulation.
    Trends Biochem Sci. 1995 Mar;20(3):113-6 PMID: 7709429
  11. The 62- and 80-kDa subunits of transcription factor IIH mediate the interaction with Epstein-Barr virus nuclear protein 2.
    Proc Natl Acad Sci U S A. 1995 Apr 11;92(8):3259-63 PMID: 7724549
  12. Excision repair in mammalian cells.
    J Biol Chem. 1995 Jul 7;270(27):15915-8 PMID: 7608140
  13. The hepatitis B virus HBx protein is a dual specificity cytoplasmic activator of Ras and nuclear activator of transcription factors.
    EMBO J. 1995 Oct 2;14(19):4747-57 PMID: 7588604
  14. Hepatitis B virus HBx protein deregulates cell cycle checkpoint controls.
    Proc Natl Acad Sci U S A. 1995 Nov 21;92(24):11215-9 PMID: 7479968
  15. SSL2, a suppressor of a stem-loop mutation in the HIS4 leader encodes the yeast homolog of human ERCC-3.
    Cell. 1992 Jun 12;69(6):1031-42 PMID: 1318786
  16. The woodchuck hepatitis virus X gene is important for establishment of virus infection in woodchucks.
    J Virol. 1993 Mar;67(3):1218-26 PMID: 8437213
  17. Hepatitis B virus transactivator HBx uses a tumour promoter signalling pathway.
    Nature. 1993 Feb 25;361(6414):742-5 PMID: 8441471
  18. Multifunctional RNA polymerase II initiation factor delta from rat liver. Relationship between carboxyl-terminal domain kinase, ATPase, and DNA helicase activities.
    J Biol Chem. 1993 Aug 15;268(23):17300-8 PMID: 8394338
  19. General initiation factors for RNA polymerase II.
    Annu Rev Biochem. 1993;62:161-90 PMID: 8352588
  20. Transactivation by hepatitis B virus X protein is promiscuous and dependent on mitogen-activated cellular serine/threonine kinases.
    Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):8078-82 PMID: 8367466
  21. Dual roles of a multiprotein complex from S. cerevisiae in transcription and DNA repair.
    Cell. 1993 Dec 31;75(7):1379-87 PMID: 8269516
  22. Induction of the DNA-binding activity of c-jun/c-fos heterodimers by the hepatitis B virus transactivator pX.
    Mol Cell Biol. 1994 Feb;14(2):989-98 PMID: 7507209
  23. Transcription initiation by RNA polymerase II does not require hydrolysis of the beta-gamma phosphoanhydride bond of ATP.
    EMBO J. 1994 Jan 15;13(2):391-9 PMID: 8313884
  24. Hepatitis B virus X protein inhibits p53 sequence-specific DNA binding, transcriptional activity, and association with transcription factor ERCC3.
    Proc Natl Acad Sci U S A. 1994 Mar 15;91(6):2230-4 PMID: 8134379
  25. Dual role of TFIIH in DNA excision repair and in transcription by RNA polymerase II.
    Nature. 1994 Apr 21;368(6473):769-72 PMID: 8152490
  26. Transcription factors IIE and IIH and ATP hydrolysis direct promoter clearance by RNA polymerase II.
    Cell. 1994 Apr 8;77(1):145-56 PMID: 8156590
  27. Transcriptional activation: a complex puzzle with few easy pieces.
    Cell. 1994 Apr 8;77(1):5-8 PMID: 8156597
  28. The hepatitis B virus transactivator protein, HBx, interacts with single-stranded DNA (ssDNA). Biochemical characterizations of the HBx-ssDNA interactions.
    J Biol Chem. 1996 Jun 28;271(26):15443-50 PMID: 8663128
  29. Hepatocellular carcinoma and hepatitis B virus. A prospective study of 22 707 men in Taiwan.
    Lancet. 1981 Nov 21;2(8256):1129-33 PMID: 6118576
  30. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
    Gene. 1988 Jul 15;67(1):31-40 PMID: 3047011
  31. A presumed DNA helicase encoded by ERCC-3 is involved in the human repair disorders xeroderma pigmentosum and Cockayne's syndrome.
    Cell. 1990 Aug 24;62(4):777-91 PMID: 2167179
  32. HBV X protein alters the DNA binding specificity of CREB and ATF-2 by protein-protein interactions.
    Science. 1991 May 10;252(5007):842-4 PMID: 1827531
  33. CTD kinase associated with yeast RNA polymerase II initiation factor b.
    Cell. 1991 Dec 20;67(6):1223-30 PMID: 1836979
  34. Binding of basal transcription factor TFIIH to the acidic activation domains of VP16 and p53.
    Mol Cell Biol. 1994 Oct;14(10):7013-24 PMID: 7935417
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.93.20.10578
Published
1996-10-01
Pages
10578-83
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC38195
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.93.20.10578 PMC Full Text View journal details
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