Abstract
The Streptococcus gordonii (Challis) glucosyltransferase-encoding determinant gtfG is regulated by the product of the adjacent gene rgg. Results of analyses described here showed that in both S. gordonii and Escherichia coli Rgg is a positive transcriptional regulator of glucosyltransferase expression. In addition, the transcriptional start sites of both gtfG and rgg were determined.
MeSH Terms
Amino Acid Sequence
Bacterial Proteins/biosynthesis,genetics,metabolism
Base Sequence
DNA-Binding Proteins
Gene Expression Regulation, Bacterial
Glucosyltransferases/biosynthesis,genetics
Molecular Sequence Data
RNA, Bacterial/analysis
RNA, Messenger/analysis
Streptococcus/genetics
Trans-Activators
Transcription Factors/genetics,metabolism
Transcription, Genetic
Chemicals
Bacterial Proteins
DNA-Binding Proteins
RNA, Bacterial
RNA, Messenger
Trans-Activators
Transcription Factors
rgg protein, Streptococcus
Glucosyltransferases
GtfG protein, Streptococcus gordonii
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sulavik M C
Department of Microbiology and Immunology, School of Medicine, University of Michigan, Ann Arbor 48109, USA.
Clewell D B
References (22)
22 references, click to expand
-
Bacterial adherence in the pathogenesis of endocarditis. Interaction of bacterial dextran, platelets, and fibrin.
J Clin Invest. 1978 May;61(5):1394-404
PMID: 659601
-
Transglucosidase activity of rumen strains of Streptococcus bovis. 2. Isolation and properties of dextransucrase.
Biochem J. 1959 May;72(1):42-9
PMID: 13651133
-
Streptococcus S.B.E.: A Streptococcus Associated with Subacute Bacterial Endocarditis.
J Bacteriol. 1946 Jun;51(6):717-22
PMID: 16561124
-
Complete nucleotide sequence of macrolide-lincosamide-streptogramin B-resistance transposon Tn917 in Streptococcus faecalis.
J Bacteriol. 1985 Nov;164(2):782-96
PMID: 2997130
-
Identification of a gene, rgg, which regulates expression of glucosyltransferase and influences the Spp phenotype of Streptococcus gordonii Challis.
J Bacteriol. 1992 Jun;174(11):3577-86
PMID: 1534326
-
Improved single and multicopy lac-based cloning vectors for protein and operon fusions.
Gene. 1987;53(1):85-96
PMID: 3596251
-
Role of Streptococcus mutans in human dental decay.
Microbiol Rev. 1986 Dec;50(4):353-80
PMID: 3540569
-
Regulation of expression of Streptococcus mutans genes important to virulence.
Infect Immun. 1990 Feb;58(2):464-70
PMID: 2298487
-
Effect of Tween 80 on glucosyltransferase production in Streptococcus mutans.
Appl Environ Microbiol. 1977 Aug;34(2):115-9
PMID: 907338
-
Multiple transcripts encoded by the ilvGMEDA gene cluster of Escherichia coli K-12.
J Bacteriol. 1992 Aug;174(15):4871-7
PMID: 1629149
-
Reiterative copying by E.coli RNA polymerase during transcription initiation of mutant pBR322 tet promoters.
Nucleic Acids Res. 1990 Feb 11;18(3):547-52
PMID: 2408014
-
DNA sequencing with chain-terminating inhibitors.
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7
PMID: 271968
-
Nucleotide sequence of the gelatinase gene (gelE) from Enterococcus faecalis subsp. liquefaciens.
Infect Immun. 1991 Jan;59(1):415-20
PMID: 1846126
-
Regulation of dextransucrase formation by Streptococcus sanguis.
Caries Res. 1973;7(2):89-101
PMID: 4518082
-
Infectivity of a glucan synthesis-defective mutant of Streptococcus gordonii (Challis) in a rat endocarditis model.
FEMS Microbiol Lett. 1993 Sep 15;112(3):301-5
PMID: 8224794
-
Spontaneous switching of the sucrose-promoted colony phenotype in Streptococcus sanguis.
Infect Immun. 1989 Dec;57(12):3945-8
PMID: 2530177
-
Transcription and decay of the lac messenger: role of an intergenic terminator.
J Bacteriol. 1991 Jan;173(1):28-36
PMID: 1702782
-
Inhibition of the synthesis and secretion of extracellular glucosyl- and fructosyltransferase in Streptococcus sanguis by sodium ions.
J Gen Microbiol. 1984 Jan;130(1):77-82
PMID: 6231355
-
Adherence of glucan-positive and glucan-negative streptococcal strains to normal and damaged heart valves.
J Clin Invest. 1978 Oct;62(4):805-14
PMID: 701479
-
Mutants of Streptococcus gordonii Challis over-producing glucosyltransferase.
J Gen Microbiol. 1991 Mar;137(3):483-9
PMID: 1851801
-
Sucrose-promoted accumulation of growing glucosyltransferase variants of Streptococcus gordonii on hydroxyapatite surfaces.
Infect Immun. 1991 Oct;59(10):3523-30
PMID: 1716611
-
Effects of Tween 80 and sodium fluoride on extracellular glucosyltransferase production and membrane lipids of Streptococcus mutans.
Int J Biochem. 1989;21(7):751-4
PMID: 2527172