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PMID: 8816819 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Rat hippocampal neurons express genes for both rod retinal and olfactory cyclic nucleotide-gated channels: novel targets for cAMP/cGMP function.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 93 ·No. 19 ·1996-09-17 ·Pages 10440-5

Kingston PA, Zufall F, Barnstable CJ

Abstract

Cyclic nucleotide-gated (CNG) channels are Ca(2+)-permeable, nonspecific cation channels that can be activated through direct interaction with cAMP and/or cGMP. Recent electrophysiological evidence for these channels in cultured hippocampal neurons prompted us to investigate the expression of CNG channel genes in hippocampus. PCR amplification detected the expression of transcripts for subunit 1 of both the rod photoreceptor (RCNGC1) and the olfactory receptor cell (OCNGC1) subtype of CNG channel in adult rat hippocampus. In situ hybridization detected expression of both channel subtypes in most principal neurons, including pyramidal cells of the CA1 through CA3 regions and granule cells of the dentate gyrus. From the hybridization patterns, we conclude that the two genes are colocalized in individual neurons. Comparison of the patterns of expression of type 1 cGMP-dependent protein kinase and the CNG channels suggests that hippocampal neurons can respond to changes in cGMP levels with both rapid changes in CNG channel activity and slower changes induced by phosphorylation. Future models of hippocampal function should include CNG channels and their effects on both electrical responses and intracellular Ca2+ levels.

MeSH Terms
Animals Cyclic AMP/physiology Cyclic GMP/physiology Cyclic GMP-Dependent Protein Kinases/biosynthesis Cyclic Nucleotide-Gated Cation Channels Dentate Gyrus/cytology,metabolism Hippocampus/cytology,metabolism In Situ Hybridization Ion Channel Gating Ion Channels/biosynthesis,physiology Neurons/cytology,metabolism Olfactory Pathways/physiology Polymerase Chain Reaction Pyramidal Cells/cytology,metabolism RNA Probes Rats Retinal Rod Photoreceptor Cells/physiology Transcription, Genetic
Chemicals
Cyclic Nucleotide-Gated Cation Channels Ion Channels RNA Probes Cyclic AMP Cyclic GMP-Dependent Protein Kinases Cyclic GMP
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kingston P A
Department of Ophthalmology and Visual Science, Yale University School of Medicine, New Haven, CT 06520, USA.
Zufall F
Barnstable C J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.93.19.10440
Published
1996-09-17
Pages
10440-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC38403
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.93.19.10440 PMC Full Text View journal details
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