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PMID: 8807063 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Characterization of tetracycline and erythromycin resistance determinants in Treponema denticola.

Antimicrobial agents and chemotherapy ·Vol. 40 ·No. 7 ·1996-07-00 ·Pages 1690-4

Roberts MC, Chung WO, Roe DE

Abstract

Treponema denticola isolates were evaluated for the presence of known tetracycline and erythromycin resistance determinants by Southern blot hybridization of whole-cell DNA and PCR assays. We examined all isolates available, which included 12 clinical and 4 American Type Culture Collection isolates. Two isolates carried the Tet B determinant, five isolates carried both the Tet B and Erm F determinants, seven isolates carried the Erm F determinant, and two did not carry any of the Tet or Erm determinants tested. Both the Tet B and Erm F determinants appeared to be associated with the chromosome. Neither of the two T. denticola donors tested could transfer the Tet B determinant, but three of four T. denticola tested transferred the Erm F determinant to an Enterococcus faecalis recipient. This extends the host range of both the tetB and ermF genes into the genus Treponema.

MeSH Terms
Anti-Bacterial Agents/pharmacology Base Sequence DNA, Bacterial/analysis,genetics Drug Resistance, Microbial Erythromycin/pharmacology Genes, Bacterial Humans Molecular Sequence Data Tetracycline Resistance/genetics Treponema/drug effects,genetics
Chemicals
Anti-Bacterial Agents DNA, Bacterial Erythromycin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Roberts M C
Department of Pathobiology, University of Washington, Seattle 98195-7238, USA. marilynr@u.washington.edu
Chung W O
Roe D E
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.40.7.1690
Email
marilynr@u.washington.edu
Published
1996-07-00
Pages
1690-4
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC163396
Subset
IM
Grants
NIAID NIH HHS · AI24136 · United States
NIDCR NIH HHS · DE10913 · United States
NIDCR NIH HHS · T32 DE07063 · United States
Databases
GENBANK
S83213
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1128/AAC.40.7.1690 PMC Full Text View journal details
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