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PMID: 2291670 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Detection of erythromycin resistance by the polymerase chain reaction using primers in conserved regions of erm rRNA methylase genes.

Antimicrobial agents and chemotherapy ·Vol. 34 ·No. 10 ·1990-10-00 ·Pages 2024-6

Arthur M, Molinas C, Mabilat C, Courvalin P

Abstract

Genes belonging to different erm DNA hybridization classes were selectively amplified by polymerase chain reaction with a pair of oligonucleotides that corresponded to conserved amino acid motifs in known ERM methylases. Identification of the resistance mechanism was possible despite substantial nucleotide sequence diversity among the erythromycin resistance genes.

MeSH Terms
Amino Acid Sequence Bacteria/drug effects,genetics Codon Drug Resistance, Microbial Erythromycin/pharmacology Molecular Sequence Data Polymerase Chain Reaction/drug effects
Chemicals
Codon Erythromycin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Arthur M
Unité des Agents Antibactériens, Institut Pasteur, Paris, France.
Molinas C
Mabilat C
Courvalin P
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22 references, click to expand
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.34.10.2024
Published
1990-10-00
Pages
2024-6
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC171984
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.34.10.2024 PMC Full Text View journal details
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